Antibiotics Resistance in Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014-2019.

() is a zoonotic pathogen that showed growing resistance to antibiotics. No descriptive analysis highlights the threat of antimicrobial-resistant (AMR) of among livestock in the United Arab Emirates (UAE). Herein, we conducted phenotypic and genotypic resistance studies on isolates from livestock samples in the Emirate of Abu Dhabi based on routine diagnosis between the periods 2014-2019. Bacterial culture and disk diffusion methods were used for bacterial isolation and phenotypic resistance analysis. Resistance mechanism was studied by PCR targeting the most commonly resistance genes: ampicillin ( , , and ), tetracyclines ( and , co-trimoxazole [sulfamethoxazole (, , and ) + trimethoprim ( and ], aminoglycosides [, and aac(3)-IV], and fluoroquinolones ( and ). Analysis of 165 isolates showed resistant to ampicillin, tetracycline, co-trimoxazole, gentamicin, and enrofloxacin by 157/165 (95.4%), 154/165 (93.6%), 141/165 (86%), 139/165 (85%), and 135/165 (82.7%), respectively. Predominant resistance gene/s detected by PCR were (119/160, 72%) and (154/160, 96.3%) for ampicillin; (162/164, 98.8%) and (112/164, 68.3%) for tetracyclines; (156/164, 95%), (138/164, 84%), and (74/164, 44.5%) for co-trimoxazole; (134/164, 82.1%) and (161/164, 98.2%) for aminoglycosides; and (61/61, 100%) for enrofloxacin. Both phenotypic and genotypic analyses revealed that all isolates were multidrug-resistant (resistance to 3, 4, and 5 antibiotics classes by 3.6%, 57.6%, and 38.8%, respectively) carrying one or more resistance gene/s for the same antibiotic. PCR profiling confirmed the presence of resistance genes corresponding to their antibiotic profile. Results of the study will highlight the knowledge based on AMR related to livestock in UAE that may call for interventions.