长链非编码 RNA SNHG20 通过 miR-140-5p-ADAM10 轴促进宫颈癌中的细胞增殖和侵袭。
LncRNA SNHG20 promotes cell proliferation and invasion via miR-140-5p-ADAM10 axis in cervical cancer.
机构信息
Department of Gynecology, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan, 453100, PR China.
Department of Gynecology, The First Affiliated Hospital of Xinxiang Medical University Weihui, Henan, 453100, PR China.
出版信息
Biomed Pharmacother. 2018 Jun;102:749-757. doi: 10.1016/j.biopha.2018.03.024. Epub 2018 Apr 5.
BACKGROUND
Recent studies highlight the crucial regulatory roles of long non-coding RNAs (lncRNAs) in carcinogenesis. However, involvement of the lncRNA SNHG20 in cervical cancer progression remains unclear.
METHODS
The expression of SNHG20 and miR-140-5p was determined in cervical cancer. Gain or loss of function assays were used to explore the roles of SNHG20 and miR-140-5p in cervical cancer cells. Luciferase assay and Western blot were used to explore the underlying mechanisms of SNHG20 and miR-140-5p in cervical cancer progression.
RESULTS
QRT-PCR showed that SNHG20 expression was significantly increased in cervical cancer. MiR-140-5p acted as a downstream target of SNHG20. SNHG20 inhibition or miR-140-5p overexpression reduced cervical cancer cells proliferation and invasion ability. Furthermore, we identified that ADAM10 could act as a potential target of miR-140-5p. MEK/ERK signaling could be inhibited by miR-140-5p mimics in cervical cancer cells. In addition, ADAM10 overexpression abrogated the effect of miR-140-5p mimics on cervical cancer cells proliferation and invasion.
CONCLUSIONS
Our study demonstrated that SNHG20 could function as an oncogenic lncRNA by regulating miR-140-5p-ADAM10 axis and MEK/ERK signaling pathway in cervical cancer.
背景
最近的研究强调了长非编码 RNA（lncRNA）在致癌作用中的关键调节作用。然而，lncRNA SNHG20 在前宫颈癌进展中的作用尚不清楚。
方法
测定宫颈癌中 SNHG20 和 miR-140-5p 的表达。采用 gain-或 loss-of-function 检测方法探讨 SNHG20 和 miR-140-5p 在宫颈癌细胞中的作用。荧光素酶报告基因检测和 Western blot 检测用于探讨 SNHG20 和 miR-140-5p 在宫颈癌进展中的潜在机制。
结果
实时定量 PCR 显示 SNHG20 在宫颈癌中的表达显著增加。miR-140-5p 是 SNHG20 的下游靶点。SNHG20 抑制或 miR-140-5p 过表达可降低宫颈癌细胞的增殖和侵袭能力。此外，我们发现 ADAM10 可以作为 miR-140-5p 的潜在靶点。miR-140-5p 模拟物可抑制宫颈癌细胞中的 MEK/ERK 信号通路。此外，ADAM10 的过表达可消除 miR-140-5p 模拟物对宫颈癌细胞增殖和侵袭的影响。
结论
本研究表明，SNHG20 可通过调节 miR-140-5p-ADAM10 轴和 MEK/ERK 信号通路在宫颈癌中发挥致癌 lncRNA 的作用。
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