巨大芽孢杆菌通过基因网络适应酸性胁迫条件：全基因组转录组分析的见解。
Bacillus megaterium adapts to acid stress condition through a network of genes: Insight from a genome-wide transcriptome analysis.
机构信息
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, 785013, India.
Department of Life-Sciences, Dibrugarh University, Dibrugarh, 786004, Assam, India.
出版信息
Sci Rep. 2018 Oct 31;8(1):16105. doi: 10.1038/s41598-018-34221-0.
RNA-seq analysis of B. megaterium exposed to pH 7.0 and pH 4.5 showed differential expression of 207 genes related to several processes. Among the 207 genes, 11 genes displayed increased transcription exclusively in pH 4.5. Exposure to pH 4.5 induced the expression of genes related to maintenance of cell integrity, pH homeostasis, alternative energy generation and modification of metabolic processes. Metabolic processes like pentose phosphate pathway, fatty acid biosynthesis, cysteine and methionine metabolism and synthesis of arginine and proline were remodeled during acid stress. Genes associated with oxidative stress and osmotic stress were up-regulated at pH 4.5 indicating a link between acid stress and other stresses. Acid stress also induced expression of genes that encoded general stress-responsive proteins as well as several hypothetical proteins. Our study indicates that a network of genes aid B. megaterium G18 to adapt and survive in acid stress condition.
在 pH 值为 7.0 和 pH 值为 4.5 的条件下培养巨大芽孢杆菌，通过 RNA-seq 分析发现，有 207 个与多种过程相关的基因呈现差异性表达。在这 207 个基因中，有 11 个基因仅在 pH 值为 4.5 的条件下转录增强。暴露在 pH 值为 4.5 的条件下，会诱导与维持细胞完整性、pH 值稳态、替代能量产生以及代谢过程修饰相关的基因表达。在酸性胁迫下，戊糖磷酸途径、脂肪酸生物合成、半胱氨酸和蛋氨酸代谢以及精氨酸和脯氨酸的合成等代谢过程发生重塑。与氧化应激和渗透应激相关的基因在 pH 值为 4.5 时上调，表明酸胁迫与其他应激之间存在关联。酸胁迫还诱导了编码一般应激反应蛋白和几个假定蛋白的基因表达。我们的研究表明，一组基因网络帮助巨大芽孢杆菌 G18 在酸性胁迫条件下适应和存活。
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