寻找一种实用的多粘菌素药敏试验方法：我们是否通过回到未来找到了它？
The Search for a Practical Method for Colistin Susceptibility Testing: Have We Found It by Going Back to the Future?
机构信息
Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, New York, USA
出版信息
J Clin Microbiol. 2019 Jan 30;57(2). doi: 10.1128/JCM.01608-18. Print 2019 Feb.
Abstract
Polymyxins are relied upon for the treatment of carbapenem-resistant Gram-negative bacterial infections, but polymyxin resistance is increasing. Only broth microdilution is recommended for polymyxin susceptibility testing, but this method is impractical for most clinical microbiology laboratories. An article in this issue of the (P. J. Simner, Y. Bergman, M. Trejo, A. A. Roberts, R. Marayan, T. Tekle, S. Campeau, A. Kazmi, D. Bell, S. Lewis, P. D. Tamma, R. Humphries, and J. A. Hindler, J Clin Microbiol 57:e01163-18, 2019, https://doi.org/10.1128/JCM.01163-18) found that colistin broth disk elution, a method that requires only colistin disks and broth, had excellent performance compared to broth microdilution for all strains except -positive strains.
摘要
黏菌素用于治疗碳青霉烯类耐药革兰氏阴性菌感染，但黏菌素耐药性正在增加。仅推荐肉汤微量稀释法用于黏菌素药敏试验，但该方法对大多数临床微生物学实验室来说不切实际。本期《临床微生物学杂志》（P. J. Simner、Y. Bergman、M. Trejo、A. A. Roberts、R. Marayan、T. Tekle、S. Campeau、A. Kazmi、D. Bell、S. Lewis、P. D. Tamma、R. Humphries 和 J. A. Hindler，J Clin Microbiol 57:e01163-18, 2019, https://doi.org/10.1128/JCM.01163-18）发现，仅需黏菌素药敏纸片和肉汤的黏菌素肉汤纸片洗脱法，除 -阳性 菌株外，与肉汤微量稀释法相比，对所有菌株均具有出色的性能。
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2
Chromosomally Encoded in Colistin-Nonsusceptible Pseudomonas aeruginosa.
Antimicrob Agents Chemother. 2018 Jul 27;62(8). doi: 10.1128/AAC.00679-18. Print 2018 Aug.
3
Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program.
PLoS One. 2018 Apr 2;13(4):e0195281. doi: 10.1371/journal.pone.0195281. eCollection 2018.
4
Rapid Polymyxin NP test for the detection of polymyxin resistance mediated by the mcr-1/mcr-2 genes.
Diagn Microbiol Infect Dis. 2018 Jan;90(1):7-10. doi: 10.1016/j.diagmicrobio.2017.09.012. Epub 2017 Sep 22.
5
New agents for the treatment of infections with Gram-negative bacteria: restoring the miracle or false dawn?
Clin Microbiol Infect. 2017 Oct;23(10):704-712. doi: 10.1016/j.cmi.2017.09.001. Epub 2017 Sep 8.
6
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7
Multicenter Clinical and Molecular Epidemiological Analysis of Bacteremia Due to Carbapenem-Resistant Enterobacteriaceae (CRE) in the CRE Epicenter of the United States.
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8
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9
Dosing guidance for intravenous colistin in critically-ill patients.
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