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模拟马桶水接种艰难梭菌孢子后,马桶喷溅气溶胶生成率及环境污染情况。

Toilet plume aerosol generation rate and environmental contamination following bowl water inoculation with Clostridium difficile spores.

机构信息

Department of Occupational and Environmental Health, University of Oklahoma College of Public Health, Oklahoma City, OK, USA.

Department of Occupational and Environmental Health, University of Oklahoma College of Public Health, Oklahoma City, OK, USA.

出版信息

Am J Infect Control. 2019 May;47(5):515-520. doi: 10.1016/j.ajic.2018.11.009. Epub 2018 Dec 14.

Abstract

INTRODUCTION

Clostridium difficile is the leading cause of health care-associated gastric illness. Environmental contamination with C difficile spores is a risk factor for contact transmission, and toilet flushing causes such contamination. This work explores toilet contamination persistence and environmental contamination produced over a series of flushes after contamination.

METHODS

A flushometer toilet was seeded with C difficile spores in a sealed chamber. The toilet was flushed 24times, with postflush bowl water samples and settle plates periodically collected for culturing and counting. Air samples were collected after each of 12 flushes using rotating plate impactors.

RESULTS

Spores were present in bowl water even after 24 flushes. Large droplet spore deposition accumulated over the 24-flush period. Droplet nuclei spore bioaerosol was produced over at least 12 flushes.

CONCLUSIONS

Toilets contaminated with C difficile spores are a persistent source of environmental contamination over an extended number of flushes.

摘要

简介

艰难梭菌是导致医疗保健相关胃部疾病的主要原因。艰难梭菌孢子对环境的污染是接触传播的一个风险因素,而马桶冲水会导致这种污染。这项工作探索了在污染后一系列冲水中马桶污染的持久性和产生的环境污染。

方法

在密封室内,使用冲力水箱式马桶接种艰难梭菌孢子。马桶被冲洗了 24 次,定期收集冲后马桶水样本和沉降板进行培养和计数。在 12 次冲水后,使用旋转平板撞击器收集空气样本。

结果

即使经过 24 次冲水,孢子仍存在于马桶水中。大量的液滴孢子沉积在 24 次冲水期间积累。在至少 12 次冲水中产生了飞沫核孢子生物气溶胶。

结论

被艰难梭菌孢子污染的马桶在多次冲水后会持续成为环境污染的来源。

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