局部进展期结直肠癌患者白细胞介素-8信使核糖核酸的表达
Interleukin-8 mRNA Expression in Locally Advanced Colorectal Cancer Patients.
作者信息
Bărbălan A, Streața Ioana, Ivan Elena Tatiana, Cherciu Irina, Șurlin V, Ioana M, Săftoiu A
机构信息
PhD student, University of Medicine and Pharmacy Craiova, Craiova, Romania.
Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova, Craiova, Romania.
出版信息
Curr Health Sci J. 2017 Jul-Sep;43(3):209-213. doi: 10.12865/CHSJ.43.03.04. Epub 2017 Sep 28.
PURPOSE
Interleukin-8 (IL-8) has been proven to promote progression of malignant tumours and control angiogenesis processes. We aim to determine and compare interleukin-8 (IL-8) gene expression level in colorectal tumors (CCR) and peritumoral samples obtained through endoscopic biopsy.
MATERIAL AND METHODS
Total mRNA was obtained from both tumoral and peritumoral tissue samples collected from patients diagnosed with colorectal cancer. Through Quantitative Real Time PCR, IL-8 gene expression was assessed in both pathologic tissue and adjacent normal mucosa.
RESULTS
In our cohort, IL8 expression was higher in adjacent normal mucosa than in tumoral tissue, in all the samples. Further studies on larger groups are required to validate our results.
目的
白细胞介素-8（IL-8）已被证明可促进恶性肿瘤进展并控制血管生成过程。我们旨在确定并比较通过内镜活检获得的结直肠肿瘤（CCR）和瘤周样本中白细胞介素-8（IL-8）基因的表达水平。
材料与方法
从诊断为结直肠癌的患者收集的肿瘤组织和瘤周组织样本中获取总mRNA。通过定量实时PCR，评估病理组织和相邻正常黏膜中IL-8基因的表达。
结果
在我们的队列中，所有样本中相邻正常黏膜中的IL8表达均高于肿瘤组织。需要对更大的群体进行进一步研究以验证我们的结果。
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