PTEN、NF-κB、WWP2、p53和c-Myc在B细胞淋巴瘤不同亚型及反应性滤泡增生中的表达失调。
Dysregulated expressions of PTEN, NF-κB, WWP2, p53 and c-Myc in different subtypes of B cell lymphoma and reactive follicular hyperplasia.
作者信息
Fu Huan, Jin Chenghao, Zhu Qingxiu, Liu Tingting, Ke Bo, Li Anan, Zhang Tingting
机构信息
Department of Hematology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University Nanchang 330006, Jiangxi, China.
Key Biologic Laboratory of Blood Tumor Cell of Jiangxi Province Nanchang 330006, Jiangxi, China.
出版信息
Am J Transl Res. 2019 Feb 15;11(2):1092-1101. eCollection 2019.
This study aimed to investigate the value of PTEN, NF-κB, WWP2, p53 and c-Myc expressions in distinguishing B cell lymphomas from reactive follicular hyperplasia (RFH), and their abilities to discriminate different B cell lymphoma subtypes. Lymphoma tissue samples were obtained from 30 follicular lymphoma (FL) patients, 30 germinal center B-cell like (GCB) diffuse large B cell lymphoma (DLBCL) patients, 30 non-GCB DLBCL patients and 30 Burkitt's lymphoma (BL) patients. And hyperplasia tissue samples were obtained from and 30 RFH patients. Immunohistochemistry was used to quantify the expressions of PTEN, NF-κB, WWP2, P53 and c-Myc. PTEN expression was elevated in GCB DLBCL and BL compared with RFH, and in GCB DLBCL, non-GCB DLBCL and BL than that in FL; WWP2 expression was higher in FL, GCB DLBCL, non-GCB DLBCL and BL compared with RFH; p53 expression increased in non-GCB DLBCL compared with RFH, and in BL compared with RFH, FL or GCB DLBCL; c-Myc expression was higher in GCB DLBCL, non-GCB DLBCL and BL compared with RFH; c-Myc expression was elevated in GCB DLBCL, non-GCB DLBCL and BL compared with FL. Additionally, PTEN negatively correlated with p53 expression in FL and CGB DLBCL, whereas NF-κB negatively correlated with WWP2 in GCB DLBCL, but positively associated with PTEN in RFH and c-Myc in BL. PTEN, WWP2, p53 and c-Myc expressions might be served as biomarkers for identification of B cell lymphomas from RFH as well as distinguishing different B cell lymphoma subtypes.
本研究旨在探讨PTEN、NF-κB、WWP2、p53和c-Myc表达在鉴别B细胞淋巴瘤与反应性滤泡增生（RFH）中的价值，及其区分不同B细胞淋巴瘤亚型的能力。从30例滤泡性淋巴瘤（FL）患者、30例生发中心B细胞样（GCB）弥漫性大B细胞淋巴瘤（DLBCL）患者、30例非GCB DLBCL患者和30例伯基特淋巴瘤（BL）患者中获取淋巴瘤组织样本。并从30例RFH患者中获取增生组织样本。采用免疫组织化学法对PTEN、NF-κB、WWP2、P53和c-Myc的表达进行定量分析。与RFH相比，GCB DLBCL和BL中PTEN表达升高，且GCB DLBCL、非GCB DLBCL和BL中的PTEN表达高于FL；与RFH相比，FL、GCB DLBCL、非GCB DLBCL和BL中WWP2表达更高；与RFH相比，非GCB DLBCL中p53表达增加，与RFH、FL或GCB DLBCL相比，BL中p53表达增加；与RFH相比，GCB DLBCL、非GCB DLBCL和BL中c-Myc表达更高；与FL相比，GCB DLBCL、非GCB DLBCL和BL中c-Myc表达升高。此外，在FL和CGB DLBCL中PTEN与p53表达呈负相关，而在GCB DLBCL中NF-κB与WWP2呈负相关，但在RFH中与PTEN呈正相关，在BL中与c-Myc呈正相关。PTEN、WWP2、p53和c-Myc表达可能作为鉴别RFH来源的B细胞淋巴瘤以及区分不同B细胞淋巴瘤亚型的生物标志物。
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