端粒长度与物质使用障碍的关联：系统评价和荟萃分析方案。

The association of telomere length with substance use disorders: systematic review and meta-analysis protocol.

机构信息

Unidad de Docencia, Investigación y Formación en Salud Mental (UDIF-SM), Servicio Murciano de Salud, c/ Lorca, n° 58, 30120, Murcia, Spain.

CIBER de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain.

出版信息

Syst Rev. 2019 Dec 1;8(1):298. doi: 10.1186/s13643-019-1199-x.

DOI:10.1186/s13643-019-1199-x

PMID:31787100

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6886210/

Abstract

BACKGROUND

The present protocol was designed for a systematic review and meta-analysis aimed at determining the association of telomere length with substance use disorders with the exclusion of nicotine addiction, and to identify potential moderators of the effect of telomere length. Such methodological information may provide guidance to improve the quality of future research on this important topic.

METHODS

Potential studies will be identified through electronic databases (PubMed/MEDLINE, EMBASE, PsycINFO, and Web of Science) up from inception onwards. The inclusion criteria will include published or unpublished observational studies (cohort, case-control, and cross-sectional studies) reporting telomere length in adult patients with substance use disorder compared with a control group. Non-human studies or other study designs such as reviews, case-only, family-based, and/or population studies with only healthy participants will be excluded, as well as those focused solely on nicotine addiction. The main outcome will be telomere length in adults with substance use disorder (primary) and, specifically, in those with alcohol use disorder (secondary). Two investigators will independently evaluate the preselected studies for possible inclusion and will extract data following a standardized protocol. Disagreements will be resolved by consensus. The risk of bias of all included studies will be assessed using the Newcastle-Ottawa Quality Assessment Scale for non-randomized studies. Data will be converted into standardized mean differences as effect size index, and random-effects models will be used for the meta-analysis. Cochran's Q statistic, I index, and visual inspection of the forest plot will be used to verify study heterogeneity. Subgroup analyses and meta-regressions will be conducted to ascertain heterogeneity. Several sensitivity analyses will be conducted to address the influence of potential confounding factors. Publication bias will be examined using the "funnel plot" method with Duval and Tweedie's trim-and-fill method and Egger test.

DISCUSSION

This systematic review will assess the association of telomere length with substance use disorders aside from nicotine addiction.

SYSTEMATIC REVIEW REGISTRATION

PROSPERO registration number CRD42019119785.

摘要

背景

本方案旨在进行系统评价和荟萃分析，以确定端粒长度与物质使用障碍的关联，排除尼古丁成瘾，并确定端粒长度效应的潜在调节因素。这种方法学信息可能为改善未来关于这一重要主题的研究质量提供指导。

方法

将通过电子数据库（PubMed/MEDLINE、EMBASE、PsycINFO 和 Web of Science）从创建之初开始识别潜在的研究。纳入标准将包括发表或未发表的观察性研究（队列、病例对照和横断面研究），报告与对照组相比，成人物质使用障碍患者的端粒长度。将排除非人类研究或其他研究设计，如综述、仅病例、基于家庭的和/或仅健康参与者的人群研究，以及仅关注尼古丁成瘾的研究。主要结局将是成人物质使用障碍患者（主要）和特别是酒精使用障碍患者（次要）的端粒长度。两名调查员将独立评估预先选择的可能纳入的研究，并按照标准化方案提取数据。意见分歧将通过协商解决。使用非随机研究的纽卡斯尔-渥太华质量评估量表评估所有纳入研究的偏倚风险。将数据转换为标准化均数差作为效应量指标，并使用随机效应模型进行荟萃分析。将使用 Cochran's Q 统计量、I 指数和森林图的视觉检查来验证研究异质性。将进行亚组分析和荟萃回归以确定异质性。将进行几项敏感性分析以解决潜在混杂因素的影响。将使用“漏斗图”方法结合 Duval 和 Tweedie 的修剪和填充方法以及 Egger 检验来检查发表偏倚。