Sonic Hedgehog 调控骨愈合。
Sonic Hedgehog Regulates Bone Fracture Healing.
机构信息
Division of Histology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu Hokkaido 061-0293, Japan.
Division of Reconstructive Surgery for Oral and Maxillofacial Region, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu Hokkaido 061-0293, Japan.
出版信息
Int J Mol Sci. 2020 Jan 20;21(2):677. doi: 10.3390/ijms21020677.
Bone fracture healing involves the combination of intramembranous and endochondral ossification. It is known that Indian hedgehog (Ihh) promotes chondrogenesis during fracture healing. Meanwhile, Sonic hedgehog (Shh), which is involved in ontogeny, has been reported to be involved in fracture healing, but the details had not been clarified. In this study, we demonstrated that Shh participated in fracture healing. Six-week-old Sprague-Dawley rats and Gli-CreER; tdTomato mice were used in this study. The right rib bones of experimental animals were fractured. The localization of Shh and Gli1 during fracture healing was examined. The localization of Gli1 progeny cells and osterix (Osx)-positive cells was similar during fracture healing. Runt-related transcription factor 2 (Runx2) and Osx, both of which are osteoblast markers, were observed on the surface of the new bone matrix and chondrocytes on day seven after fracture. Shh and Gli1 were co-localized with Runx2 and Osx. These findings suggest that Shh is involved in intramembranous and endochondral ossification during fracture healing.
骨愈合涉及膜内和软骨内骨化的结合。已知，印度刺猬（Ihh）在骨折愈合过程中促进软骨生成。同时，参与个体发生的 Sonic hedgehog（Shh）已被报道参与骨折愈合，但细节尚未阐明。在这项研究中，我们证明了 Shh 参与了骨折愈合。本研究使用了 6 周龄的 Sprague-Dawley 大鼠和 Gli-CreER；tdTomato 小鼠。实验动物的右侧肋骨骨折。检查了 Shh 和 Gli1 在骨折愈合过程中的定位。Gli1 祖细胞和骨钙素（Osx）阳性细胞在骨折愈合过程中的定位相似。成骨细胞标志物 Runt 相关转录因子 2（Runx2）和 Osx 在骨折后第 7 天观察到在新骨基质和软骨细胞的表面上。Shh 和 Gli1 与 Runx2 和 Osx 共定位。这些发现表明 Shh 参与了骨折愈合过程中的膜内和软骨内骨化。
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