急性原发性闭角型青光眼患者房水中 TGF-β1、TGF-β2、MMP-2 和 TIMP-1 水平升高。

Higher TGF-β1, TGF-β2, MMP-2, and TIMP-1 Levels in the Aqueous Humor of Patients with Acute Primary Angle Closure.

机构信息

Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.

Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China,

出版信息

Ophthalmic Res. 2021;64(1):62-67. doi: 10.1159/000507762. Epub 2020 Apr 8.

DOI:10.1159/000507762

PMID:32259818

Abstract

PURPOSE

To assess the quantitative differences in the levels of members of the transforming growth factor (TGF-β), matrix metalloproteinase (MMP), and tissue inhibitor of MMP (TIMP) families in the aqueous humor (AH) between patients with acute primary angle closure (APAC) and those with cataract only.

METHODS

AH samples were collected from 26 patients with APAC and cataract as well as 26 patients with age-related cataract only. Multiplex assays were used to measure the concentrations of TGF-β1, TGF-β2, and TGF-β3; MMP-1, MMP-2, MMP-7, MMP-9, and MMP-10; and TIMP-1 and TIMP-2.

RESULTS

The concentrations of TGF-β1, TGF-β2, MMP-2, (p < 0.001), and TIMP-1 were significantly higher (all p < 0.001) in AH samples from patients with APAC versus cataract only. Conversely, the AH concentrations of MMP-7 (p = 0.524), MMP-9 (p = 0.103), MMP-10 (p = 0.111), and TIMP-2 (p = 0.059) did not significantly differ between the groups. The concentrations of TGF-β3 and MMP-1 were below the respective detection limits in most AH samples.

CONCLUSION

The AH levels of TGF-β1, TGF-β2, MMP-2, and TIMP-1 were elevated in APAC eyes. Such altered protein levels could induce abnormal deposition of extracellular matrix in the trabecular meshwork, resulting in an increase in aqueous outflow resistance and, thereby, providing a possible explanation of the mechanism of residual glaucoma after cataract surgery.

摘要

目的

评估急性原发性闭角型青光眼（APAC）患者和单纯白内障患者房水中转化生长因子（TGF-β）、基质金属蛋白酶（MMP）和 MMP 组织抑制剂（TIMP）家族成员水平的定量差异。

方法

收集 26 例 APAC 合并白内障和 26 例单纯年龄相关性白内障患者的房水样本。采用多重分析测定 TGF-β1、TGF-β2 和 TGF-β3；MMP-1、MMP-2、MMP-7、MMP-9 和 MMP-10；以及 TIMP-1 和 TIMP-2 的浓度。

结果

APAC 合并白内障患者房水中 TGF-β1、TGF-β2、MMP-2（均 p < 0.001）和 TIMP-1 的浓度明显高于单纯白内障患者（均 p < 0.001）。相反，APAC 合并白内障患者房水中 MMP-7（p = 0.524）、MMP-9（p = 0.103）、MMP-10（p = 0.111）和 TIMP-2（p = 0.059）的浓度在两组间无显著差异。大多数房水样本中 TGF-β3 和 MMP-1 的浓度低于各自的检测下限。