近视患者房水和血浆中转化生长因子-β与基质金属蛋白酶的关系。

Association between transforming growth factors-β and matrix metalloproteinases in the aqueous humor and plasma in myopic patients.

机构信息

Department of Ophthalmology, Ninth People's Hospital affiliated Shanghai Jiao Tong University School of Medicine, 200011 Shanghai, China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, 200011 Shanghai, China; Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, 400016 Chongqing, China.

出版信息

J Fr Ophtalmol. 2022 Dec;45(10):1177-1183. doi: 10.1016/j.jfo.2022.05.014. Epub 2022 Nov 7.

DOI:10.1016/j.jfo.2022.05.014

PMID:36357250

Abstract

PURPOSE

To investigate the relationship between levels of transforming growth factor beta (TGF-beta), matrix metalloproteinase (MMP) and tissue inhibitors of MMP (TIMP) in the aqueous humor (AH) and plasma (PL) of myopic patients.

METHODS

Aqueous humor and plasma were collected intraoperatively from 37 myopic patients with various axial lengths (AL) during ICL surgery. The concentrations of TGF-β1, TGF-β2, TGF-β3, MMP-1, MMP-2, MMP-7, MMP-9, MMP-10, TIMP-1, TIMP-2, TIMP-3 and TIMP-4 were measured using Luminex xMAP Technology kits (Milliplex xMAP kits). The association between TGFβ and MMP/TIMP levels were analyzed based on the Spearman's correlation test or Pearson's correlation test.

RESULTS

There was a negative correlation between TGF-β1 and MMP-1, TIMP-2, TIMP-3 and TIMP-4 in the AH, and a positive correlation between TGF-β1 and MMP-1 in the PL. In the AH, levels of TGF-β2 were positively correlated with levels of TIMP-3 (r=0.441; P < 0.001). In the PL, levels of TGF-β2 also correlated positively with levels of TIMP-3 (r=0.427; P < 0.001)).

CONCLUSION

The level of TGF-β2 was the highest among TGF-βs in the AH. A consistent positive correlation between TGF-β2 and TIMP-3 was found both in the AH and PL, indicating that systemic levels of TGF-β2 and MMPs/TIMPs may also play a role in myopic progression.

摘要

目的

研究转化生长因子-β（TGF-β）、基质金属蛋白酶（MMP）及其组织抑制剂（TIMP）在近视患者房水（AH）和血浆（PL）中的水平关系。

方法

在 ICL 手术中，从 37 名具有不同眼轴（AL）的近视患者中收集房水和血浆。使用 Luminex xMAP 技术试剂盒（Milliplex xMAP 试剂盒）测量 TGF-β1、TGF-β2、TGF-β3、MMP-1、MMP-2、MMP-7、MMP-9、MMP-10、TIMP-1、TIMP-2、TIMP-3 和 TIMP-4 的浓度。根据 Spearman 相关检验或 Pearson 相关检验分析 TGFβ 与 MMP/TIMP 水平之间的关系。

结果

在 AH 中，TGF-β1 与 MMP-1、TIMP-2、TIMP-3 和 TIMP-4 呈负相关，与 MMP-1 在 PL 中呈正相关。在 AH 中，TGF-β2 水平与 TIMP-3 水平呈正相关（r=0.441；P<0.001）。在 PL 中，TGF-β2 水平也与 TIMP-3 水平呈正相关（r=0.427；P<0.001）。