长链非编码RNA SNHG1通过Notch1信号通路抑制胃癌细胞增殖并促进其凋亡。
lncRNA SNHG1 suppresses gastric cancer cell proliferation and promotes apoptosis via Notch1 pathway.
作者信息
Zhang Zhu, Wang Huahong
机构信息
Digestive Department, Beijing Hospital, Beijing 100730, China.
出版信息
J BUON. 2020 Jan-Feb;25(1):302-307.
PURPOSE
To explore the influence of long non-coding ribonucleic acid (lncRNA) small nucleolar RNA host gene 1 (SNHG1) on the proliferation and apoptosis of gastric cancer cells.
METHODS
SNHG1 was knocked down using small-interfering RNAs (siRNAs) in gastric cancer cell line MGC-803 and then the changes in the expression levels of SNHG1 and Notch1 in each group of cells were evaluated via quantitative reverse transcription polymerase chain reaction (qRT-PCR).
RESULTS
After intervention of SNHG1 by siRNAs, compared with NC-siRNA group, SNHG1-siRNA group exhibited notably lowered RNA and messenger RNA (mRNA) levels of SNHG1 and Notch1 (p<0.05), a substantially lowered proliferation rate (p<0.05), a remarkably raised apoptosis rate (p<0.05) and markedly decreased protein expression levels of Notch1 and Bax (p<0.05). Additionally, the cells treated with Notch1 inhibitor had the proliferation substantially inhibited (p<0.05), while there was no notable change in the RNA expression of SNHG1 in the cells.
CONCLUSIONS
LncRNA SNHG1 can depend on the Notch1 pathway to suppress the proliferation of gastric cancer cells and promote their apoptosis.
目的
探讨长链非编码核糖核酸（lncRNA）小核仁RNA宿主基因1（SNHG1）对胃癌细胞增殖和凋亡的影响。
方法
利用小干扰RNA（siRNA）敲低胃癌细胞系MGC-803中的SNHG1，然后通过定量逆转录聚合酶链反应（qRT-PCR）评估每组细胞中SNHG1和Notch1表达水平的变化。
结果
经siRNA干预SNHG1后，与NC-siRNA组相比，SNHG1-siRNA组的SNHG1和Notch1的RNA及信使核糖核酸（mRNA）水平显著降低（p<0.05），增殖率大幅降低（p<0.05），凋亡率显著升高（p<0.05），Notch1和Bax的蛋白表达水平明显降低（p<0.05）。此外，用Notch1抑制剂处理的细胞增殖受到明显抑制（p<0.05），而细胞中SNHG1的RNA表达无明显变化。
结论
LncRNA SNHG1可通过Notch1途径抑制胃癌细胞增殖并促进其凋亡。
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