检测感染患者病房空气中和表面的 SARS-CoV-2 污染情况。

Detection of air and surface contamination by SARS-CoV-2 in hospital rooms of infected patients.

机构信息

National Centre for Infectious Diseases, Singapore, Singapore.

Tan Tock Seng Hospital, Singapore, Singapore.

出版信息

Nat Commun. 2020 May 29;11(1):2800. doi: 10.1038/s41467-020-16670-2.

DOI:10.1038/s41467-020-16670-2

PMID:32472043

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7260225/

Abstract

Understanding the particle size distribution in the air and patterns of environmental contamination of SARS-CoV-2 is essential for infection prevention policies. Here we screen surface and air samples from hospital rooms of COVID-19 patients for SARS-CoV-2 RNA. Environmental sampling is conducted in three airborne infection isolation rooms (AIIRs) in the ICU and 27 AIIRs in the general ward. 245 surface samples are collected. 56.7% of rooms have at least one environmental surface contaminated. High touch surface contamination is shown in ten (66.7%) out of 15 patients in the first week of illness, and three (20%) beyond the first week of illness (p = 0.01, χ test). Air sampling is performed in three of the 27 AIIRs in the general ward, and detects SARS-CoV-2 PCR-positive particles of sizes >4 µm and 1-4 µm in two rooms, despite these rooms having 12 air changes per hour. This warrants further study of the airborne transmission potential of SARS-CoV-2.

摘要

了解空气中的颗粒大小分布和 SARS-CoV-2 的环境污染模式对于感染预防政策至关重要。在这里，我们对 COVID-19 患者的病房内的表面和空气样本进行了 SARS-CoV-2 RNA 筛查。环境采样在 ICU 的三个空气传播感染隔离室（AIIR）和普通病房的 27 个 AIIR 中进行。共采集了 245 个表面样本。至少有一个环境表面受到污染的房间占比为 56.7%。在疾病的第一周，有 15 名患者中的 10 名（66.7%）出现了高接触表面污染，而在第一周之后，有 3 名（20%）患者出现了这种情况（p=0.01，卡方检验）。在普通病房的 27 个 AIIR 中，有三个进行了空气采样，在其中两个房间中检测到大小大于 4μm 和 1-4μm 的 SARS-CoV-2 PCR 阳性颗粒，尽管这些房间每小时有 12 次空气交换。这需要进一步研究 SARS-CoV-2 的空气传播潜力。