[Knockdown of Fez family zinc finger protein 1 antisense ribonucleic acid 1 (FEZF1-AS1) inhibits invasion and migration of esophageal squamous cell carcinoma cells by blocking JAK2/STAT3 pathway].

Objective To investigate the relationship between long chain non-coding RNA Fez family zinc finger protein 1 antisense ribonucleic acid 1 (FEZF1-AS1) and invasion, migration and Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signal pathway in esophageal squamous cell carcinoma (ESCC) cells. Methods The expression' of FEZF1-AS1 in ESCC cancer tissue and ESCC cell line was detected by real-time quantitative PCR. After knocking down the FEZF1-AS1 of KYSE150 and KYSE510 cells by the constructed lentiviral expression vector, the changes of cell cycle were detected by flow cytometry, the ability of cell proliferation by colony formation assay, the ability of cell invasion by Transwell invasion assay, the ability of cell migration by Transwell migration and scratch assay, and the protein levels of JAK2 and phosphorylated STAT3 (p-STAT3) by Western blotting. Results FEZF1-AS1 was highly expressed in ESCC cancer tissues, and FEZF1-AS1 expression in KYSE150 and KYSE510 cells were higher than that in normal esophageal squamous epithelial cells. After the knockdown of FEZF1-AS1 expression, the cell cycle and proliferation of ESCC cancer cells had no obvious change, but cell invasion and migration were significantly inhibited, and the level of JAK2 protein decreased and p-STAT3 protein increased. Conclusion The knockdown of FEZF1-AS1 blocks JAK2/STAT3 pathway and inhibits the invasion and migration of ESCC cells.