The Impact of SlyA on Cell Metabolism of : A Joint Study of Transcriptomics and Metabolomics.

SlyA is an important transcriptional regulator in (). Numerous reports have indicated the impact of SlyA on the virulence of . Less information regarding the role of SlyA in the cell metabolism of is available. To close this gap, we compared the growth kinetics of an wild-type strain to a deletion mutant strain. The data suggested that the cell growth of was impaired when abolished, indicating that SlyA might affect the cell metabolism of . To determine the role of SlyA in cell metabolism, we analyzed the metabolite profiles of in the presence or absence of using gas chromatography coupled with tandem mass spectrometry (GC-MS-MS). With the aim of appropriately interpreting the results obtained from metabolomics, a transcriptomic analysis on both the wild-type and the deletion mutant was performed. The metabolome data indicated that several glycolysis and lipid metabolism-associated pathways, including the turnover of glycerolipid, pyruvate, butanoate, and glycerophospholipid, were affected in the absence of . In addition, the mRNA levels of several genes associated with glycolysis and lipid turnover were downregulated when was deleted, including , , , , and . Collectively, these evidence suggested that SlyA affects the glycolysis and lipid turnover of at a transcriptional level. The raw data of metabolomics is available in the MetaboLights database with an access number of MTBLS1858. The raw data of transcriptome is available in the Sequence Read Archive (SRA) database with an access number of PRJNA656165.