传染性支气管炎病毒 Q1 在无特定病原体小鸡中的免疫发病机制。
Immunopathogenesis of infectious bronchitis virus Q1 in specific pathogen free chicks.
机构信息
Institute of Infection and Global Health, University of Liverpool, Leahurst, UK; Faculty of Veterinary Medicine, Diyala University, Iraq.
Institute of Infection and Global Health, University of Liverpool, Leahurst, UK.
出版信息
Microb Pathog. 2020 Dec;149:104535. doi: 10.1016/j.micpath.2020.104535. Epub 2020 Sep 24.
The immunopathogenesis of avian coronavirus, infectious bronchitis virus (IBV) Q1, was investigated in specific pathogen free chicks. Following infection, chicks exhibited respiratory clinical signs and reduced body weight. Oropharyngeal (OP) and cloacal (CL) swabs were collected at intervals and found to be RT-PCR positive, with a greater number of partial-S1 amino acid changes noted in CL swabs compared to OP swabs. In tissue samples, IBV viral load peaked 9 days post infection (dpi) in the trachea and kidneys, and 14 dpi in the proventriculus. At 28 dpi, ELISA data showed that 63% of infected chicks seroconverted. There was significantly higher mRNA up-regulation of IFN-α, TLR3, MDA5, LITAF, IL-1β and IL-6 in the trachea compared to the kidneys. Findings presented here demonstrate that this Q1 isolate induces greater lesions and host innate immune responses in chickens' tracheas compared to the kidneys.
本研究旨在探究禽冠状病毒、传染性支气管炎病毒（IBV）Q1 的免疫发病机制。在感染后，雏鸡表现出呼吸道临床症状和体重减轻。在不同时间采集了口腔（OP）和泄殖腔（CL）拭子，发现 RT-PCR 检测呈阳性，CL 拭子中 S1 部分氨基酸的变化数量明显多于 OP 拭子。在组织样本中，IBV 病毒载量在感染后 9 天（dpi）时在气管和肾脏中达到峰值，在 14 dpi 时在肌胃中达到峰值。在 28 dpi 时，ELISA 数据显示 63%的感染雏鸡发生血清转化。与肾脏相比，气管中 IFN-α、TLR3、MDA5、LITAF、IL-1β 和 IL-6 的 mRNA 上调水平显著更高。本研究结果表明，与肾脏相比，该 Q1 分离株在鸡的气管中引起更大的病变和宿主固有免疫反应。
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