Wild Bitter Melon Exerts Anti-Inflammatory Effects by Upregulating Injury-Attenuated CISD2 Expression following Spinal Cord Injury.

BACKGROUND

Spinal cord injuries (SCIs) induce secondary neuroinflammation through astrocyte reactivation, which adversely affects neuronal survival and eventually causes long-term disability. CDGSH iron sulfur domain 2 (CISD2), which has been reported to be involved in mediating the anti-inflammatory responses, can serve as a target in SCI therapy. Wild bitter melon (WBM; Linn. var. abbreviata Ser.) contains an anti-inflammatory agent called alpha-eleostearic acid (-ESA), a peroxisome proliferator-activated receptor- (PPAR-) ligand. Activated PPAR- inhibits the nuclear factor B (NF-B) signaling pathway via the inhibition of IB (inhibitor of NF-B) degradation. The role of astrocyte deactivation and CISD2 in anti-inflammatory mechanisms of WBM in acute SCIs is unknown.

MATERIALS AND METHODS

A mouse model of SCI was generated via spinal cord hemisection. The SCI mice were administered WBM intraperitoneally (500 mg/kg bodyweight). Lipopolysaccharide- (LPS-) stimulated ALT cells (astrocytes) were used as an model for studying astrocyte-mediated inflammation post-SCI. The roles of CISD2 and PPAR- in inflammatory signaling were examined using LPS-stimulated SH-SY5Y cells transfected with si-CISD2 or scramble RNA.

RESULTS

WBM mitigated the SCI-induced downregulation of CISD2, PPAR-, and IB and upregulation of glial fibrillary acidic protein (GFAP; marker of astrocyte reactivation) in the spinal cord of SCI mice. Additionally, WBM (1 g/mL) mitigated LPS-induced CISD2 downregulation. Furthermore, SH-SY5Y neural cells with CISD2 knockdown exhibited decreased PPAR- expression and augmented NF-B signaling.

CONCLUSION

To the best of our knowledge, this is the first study to report that CISD2 is an upstream modulator of the PPAR-/NF-B proinflammatory signaling pathway in neural cells, and that WBM can mitigate the injury-induced downregulation of CISD2 in SCI mice and LPS-stimulated ALT astrocytes.