CC16和IL-12在矽肺各期支气管肺泡灌洗液中的临床意义

Clinical significance of CC16 and IL-12 in bronchoalveolar lavage fluid of various stages of silicosis.

作者信息

Zhang Suhua, Jia Qiang, Song Junhua, Tan Qitao, Yu Gongchang, Guo Xinfeng, Zhang Hongxiang

机构信息

Department of Laboratory, Zibo Prevention and Treatment Hospital for Occupational Diseases, Zibo, China.

Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China.

出版信息

Ann Palliat Med. 2020 Nov;9(6):3848-3856. doi: 10.21037/apm-20-1838.

DOI:10.21037/apm-20-1838

PMID:33302652

Abstract

BACKGROUND

Identification of novel biomarkers for silicosis could be helpful for disease diagnosis and pathophysiological mechanism exploration. Our study aims to investigate the Clara cell secretory 16-kd protein (CC16) and interleukin-12 (IL-12) levels in bronchoalveolar lavage fluid (BALF) in patients with silicosis at various stages.

METHODS

The enzyme-linked immunosorbent assay (ELISA) double antibody sandwich method was used to determine the CC16 and IL-12 in BALF levels from 79 patients with silicosis of various stages. Correlation analyses were performed between CC16 and IL-12 levels, and lung function and cytological counts in patients with silicosis at various stages.

RESULTS

There were no significant differences in the BALF recovery volume, the number of cells, percentages of macrophages and lymphocytes in the alveolar lavage fluid of patients with silicosis in different stages (P>0.05); the percentage of neutrophils in stage I and stage II were higher than the control group (P<0.05) with statistically significant differences. The CC16 in BALF levels in stage I and II silicosis groups lower than the control group and stage III silicosis group with statistically significant differences (P<0.05), whereas CC16 levels in stage II silicosis group are higher than the stage I group (P<0.01). The IL-12 levels were higher than the control group (P<0.01), and the IL-12 levels in stage II and III silicosis group was higher than the stage I silicosis group (P<0.01). With the increase of the length of dust service, the CC16 and IL-12 levels decreased and showed a positive correlation between these indexes (correlation coefficient r=0.559, P<0.01). In addition, CC16 silicosis patient levels were positively correlated with FEV1/FVC and VCmax (r=0.242, 0.257; both P<0.05); IL-12 levels were negatively correlated with FEV1 and VC max (r=-0.250, -0.483; both P<0.05).

CONCLUSIONS

The CC16 and IL-12 levels may have a specific reference value for the early diagnosis of silicosis and the assessment of lung function.

摘要

背景

鉴定矽肺的新型生物标志物有助于疾病诊断和病理生理机制探索。我们的研究旨在调查不同阶段矽肺患者支气管肺泡灌洗液（BALF）中克拉拉细胞分泌蛋白16（CC16）和白细胞介素12（IL-12）的水平。

方法

采用酶联免疫吸附测定（ELISA）双抗体夹心方法测定79例不同阶段矽肺患者BALF中CC16和IL-12的水平。对不同阶段矽肺患者的CC16和IL-12水平、肺功能及细胞计数进行相关性分析。

结果

不同阶段矽肺患者BALF回收量、细胞数、肺泡灌洗液中巨噬细胞和淋巴细胞百分比差异无统计学意义（P>0.05）；Ⅰ期和Ⅱ期中性粒细胞百分比高于对照组（P<0.05），差异有统计学意义。矽肺Ⅰ期和Ⅱ期组BALF中CC16水平低于对照组和矽肺Ⅲ期组，差异有统计学意义（P<0.05），而矽肺Ⅱ期组CC16水平高于Ⅰ期组（P<0.01）。IL-12水平高于对照组（P<0.01），矽肺Ⅱ期和Ⅲ期组IL-12水平高于矽肺Ⅰ期组（P<0.01）。随着接尘工龄的增加，CC16和IL-12水平降低，且这些指标之间呈正相关（相关系数r=0.559，P<0.01）。此外，矽肺患者CC16水平与FEV1/FVC和VCmax呈正相关（r=0.242，0.257；P均<0.05）；IL-12水平与FEV1和VCmax呈负相关（r=-0.250，-0.483；P均<0.05）。