The need to investigate continuums of plastic particle diversity, brackish environments and trophic transfer to assess the risk of micro and nanoplastics on aquatic organisms.

Plastic particles are ubiquitous in marine and freshwater environments. While many studies have focused on the toxicity of microplastics (MPs) and nanoplastics (NPs) in aquatic environments there is no clear conclusion on their environmental risk, which can be attributed to a lack of standardization of protocols for in situ sampling, laboratory experiments and analyzes. There are also far more studies concerning marine environments than fresh or brackish waters despite their role in the transfer of plastics from continents to oceansWe systematically reviewed the literature for studies: (1) using plastics representative of those found in the environment in laboratory experiments, (2) on the contamination of plastic particles in the continuum between fresh and marine waters, focusing in particular on estuaries and (3) on the continuum of contamination of plastic particles between species through trophic transfer in aquatic environments. We found that the exposure of aquatic organisms in the laboratory to plastic particles collected in the environment are very scarce. Moreover, plastic exposures of estuarine species in the laboratory are generally carried out for a single salinity and a single temperature that do not reflect the fluctuating environmental conditions of estuaries. Finally, the trophic transfer of plastic particles is mainly studied in the laboratory through simple food chains which are not representative of the complexity of the trophic networks observed in the aquatic environment. We pointed out that future studies in the laboratory should include both MPs and NPs sampled in the environment and focus on the precise characterization of the composition and surface of these plastics as well as on their absorbed pollutants, additives or biofilms. Moreover, investigations must be continued concerning the toxicity of plastic particles in brackish water environments such as estuaries and the trophic transfer of plastic particles in complex food chains.