经典大鼠主动脉环模型的改良以允许血管退行性变研究。

Modifications to the classical rat aortic ring model to allow vascular degeneration studies.

机构信息

Institut de la Vision, Sorbonne Université, INSERM, CNRS, Paris, France.

出版信息

STAR Protoc. 2021 Jan 21;2(1):100281. doi: 10.1016/j.xpro.2020.100281. eCollection 2021 Mar 19.

DOI:10.1016/j.xpro.2020.100281

PMID:33532730

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7821348/

Abstract

The classical aortic ring model is well suited for deciphering pro-angiogenic processes. Here, we propose simple modifications of the standard protocol to study various anti-angiogenic processes from growth arrest to capillary degeneration. Aortic rings are cultured under basal conditions for 6 days to allow physiological vessel sprouting and then split into treatment groups to follow capillary growth or degeneration for an additional 2 days.

摘要

经典的主动脉环模型非常适合破译促血管生成过程。在这里，我们提出了对标准方案的简单修改，以研究从生长停滞到毛细血管退化的各种抗血管生成过程。主动脉环在基础条件下培养 6 天，以允许生理血管发芽，然后将其分成治疗组，再培养 2 天以观察毛细血管的生长或退化。