PrimeDesign 软件可快速简化向导 RNA 设计。
PrimeDesign software for rapid and simplified design of prime editing guide RNAs.
机构信息
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.
出版信息
Nat Commun. 2021 Feb 15;12(1):1034. doi: 10.1038/s41467-021-21337-7.
Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens. Using PrimeDesign, we construct PrimeVar, a comprehensive and searchable database that includes candidate prime editing guide RNA (pegRNA) and nicking sgRNA (ngRNA) combinations for installing or correcting >68,500 pathogenic human genetic variants from the ClinVar database. Finally, we use PrimeDesign to design pegRNAs/ngRNAs to install a variety of human pathogenic variants in human cells.
Prime 编辑 (PE) 是一种多功能的基因组编辑技术，但所需的指导 RNA 的设计比标准的基于 CRISPR 的核酸酶或碱基编辑器更为复杂。在这里，我们描述了 PrimeDesign，这是一个用户友好的端到端网络应用程序和命令行工具，用于设计 PE 实验。PrimeDesign 可用于单编辑和组合编辑应用，以及全基因组和饱和诱变筛选。使用 PrimeDesign，我们构建了 PrimeVar，这是一个全面的、可搜索的数据库，其中包括候选的 Prime 编辑引导 RNA (pegRNA) 和切口 sgRNA (ngRNA) 组合，用于安装或纠正来自 ClinVar 数据库的 >68,500 种致病人类遗传变异。最后，我们使用 PrimeDesign 设计 pegRNAs/ngRNAs，以在人类细胞中安装各种人类致病变异。
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