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从土壤中分离出的一种新细菌——新种的描述。

Description of p. nov., a new bacterium isolated from soil.

作者信息

Paek Jayoung, Bai Lu, Shin Yeseul, Kim Hongik, Kook Joong-Ki, Chang Young-Hyo

机构信息

ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Industrial bio-Materials Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004707. Epub 2021 Feb 17.

Abstract

Two strains isolated from soil samples were designated as YH-JAE5 and YH-JAE2. The isolates were facultative anaerobic, Gram-stain-variable, motile, rod-shaped bacteria. Phylogenetic analysis indicated that the isolates belonged to the genus , but the 16S rRNA gene sequence similarities were <98 % when compared with other species within the genus. Analysis of gene revealed the isolates formed a sub-cluster with . The only menaquinone identified was MK-7. The two isolates contained -diaminopimelic acid within their cell wall peptidoglycan. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipid, aminophospholipids, and lipids. The major fatty acids were C anteiso and C iso. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between isolate YH-JAE5 and the most closely related reference strain ( KCTC 3758) were 81.7, 84.8 and 23.4 %, respectively. The G+C content of the genomic DNA was 47.4 mol%. Thus, the polyphasic data revealed that YH-JAE2 (=KCTC 43239=JCM 34435) and YH-JAE5 (=KCTC 43059=JCM 33533) represent a new species. The name sp. nov. is proposed.

摘要

从土壤样本中分离出的两株菌株分别命名为YH-JAE5和YH-JAE2。这些分离株为兼性厌氧菌,革兰氏染色可变,具运动性,呈杆状。系统发育分析表明,这些分离株属于 属,但与该属内其他物种相比,其16S rRNA基因序列相似性<98%。对 基因的分析表明,这些分离株与 形成一个亚簇。鉴定出的唯一甲萘醌是MK-7。这两株分离株在其细胞壁肽聚糖中含有 -二氨基庚二酸。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂、氨基磷脂和脂质。主要脂肪酸为anteiso-C 和iso-C 。分离株YH-JAE5与最密切相关的参考菌株(KCTC 3758)之间的平均核苷酸同一性、平均氨基酸同一性和数字DNA-DNA杂交值分别为81.7%、84.8%和23.4%。基因组DNA的G+C含量为47.4mol%。因此,多相数据表明YH-JAE2(=KCTC 43239=JCM 34435)和YH-JAE5(=KCTC 43059=JCM 33533)代表一个新物种。提议命名为 新种。

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