评价正畸牙移动过程中龈沟液中白细胞介素-1β及其受体拮抗剂的比值。

Evaluation of interleukin-1 beta and the ratio of interleukin-1 beta to interleukin-1 receptor antagonist in gingival crevicular fluid during orthodontic canine retraction.

机构信息

Department of Orthodontics, Faculty of Medicine, Jagiellonian University Medical College, Kraków, Poland.

School of Dentistry, University of Siena, Italy.

出版信息

Dent Med Probl. 2021 Jan-Mar;58(1):47-54. doi: 10.17219/dmp/132954.

DOI:10.17219/dmp/132954

PMID:33756073

Abstract

BACKGROUND

Orthodontic tooth movement (OTM) is a complex phenomenon mediated by cytokines, of which interleukin-1 beta (IL‑1β) is potently involved in the remodeling of the periodontal ligament (PDL) and bone. Whether the pattern of IL‑1β release differs at the sides of tension and compression is not yet clarified.

OBJECTIVES

The aim of the present study was to evaluate the level of IL‑1β and the ratio of IL‑1β to interleukin-1 receptor antagonist (IL‑1RA) in gingival crevicular fluid (GCF) at the tension and compression sides during orthodontic canine retraction.

MATERIAL AND METHODS

Seventeen patients scheduled for orthodontic treatment with bilateral extraction of maxillary first premolars and canine retraction were enrolled. Tooth 2.3 was retracted, teeth 1.3 and 3.3 served as controls. Gingival crevicular fluid samples were collected from the tension and compression sides of each tooth at baseline (before the 1st activation - day 0) and at days 2 and 7, and then again before the 2nd activation (day 28) and at days 30 and 35. The levels of IL‑1β and IL‑RA were evaluated with the enzyme-linked immunosorbent assay (ELISA).

RESULTS

After the 1st activation, a statistically significant increase in the level of IL‑1β was observed at teeth 2.3 (p < 0.03 mesially and p < 0.05 distally) and 1.3 (p < 0.05 mesially and distally), both at the tension and compression sides. The 2nd activation resulted in a gradual increase in the IL‑1β level at both canines; however, statistical significance was reached only for tooth 2.3 (p < 0.05 mesially and p < 0.02 distally). In terms of the IL‑1β/IL‑1RA ratio, a significant increase was observed only at the compression side of the experimental tooth (p < 0.01).

CONCLUSIONS

An increase in the IL‑1β level in GCF was observed both at the tension and compression sides of the actively retracted canine 2.3 as well as the contralateral canine 1.3; a significant rise in the IL‑1β/IL‑1RA ratio was noted only at the compression side of the experimental tooth 2.3, indicating the zone of active bone resorption.

摘要

背景

正畸牙齿移动（OTM）是一种由细胞因子介导的复杂现象，其中白细胞介素-1β（IL-1β）在牙周韧带（PDL）和骨改建中起着重要作用。IL-1β释放的模式在张力侧和压力侧是否不同尚不清楚。

目的

本研究旨在评估在正畸尖牙回收过程中，牙周袋内液（GCF）中 IL-1β的水平以及 IL-1β与白细胞介素-1受体拮抗剂（IL-1RA）的比值在张力侧和压力侧的变化。

材料和方法

本研究共纳入 17 名计划接受正畸治疗的患者，均接受上颌第一前磨牙双侧拔牙和尖牙回收。2.3 号牙被回收，1.3 号牙和 3.3 号牙作为对照。在基线（第 1 次激活前-第 0 天）和第 2 天、第 7 天，以及第 2 次激活前（第 28 天）和第 30 天、第 35 天，分别从每颗牙的张力侧和压力侧采集龈沟液样本。采用酶联免疫吸附试验（ELISA）检测 IL-1β和 IL-RA 的水平。

结果

第 1 次激活后，2.3 号牙（近中 p<0.03，远中 p<0.05）和 1.3 号牙（近中 p<0.05，远中 p<0.05）的张力侧和压力侧的 IL-1β水平均有统计学意义的升高。第 2 次激活后，双侧尖牙的 IL-1β水平逐渐升高，但只有 2.3 号牙的升高具有统计学意义（近中 p<0.05，远中 p<0.02）。就 IL-1β/IL-1RA 比值而言，仅在实验牙的压力侧观察到显著升高（p<0.01）。