饥饿细胞的细胞内代谢组。

The intracellular metabolome of starving cells.

机构信息

Metabolomics and Cell Biology Platforms, Gustave Roussy Comprehensive Cancer Institute, Université Paris Saclay, Villejuif, France; Centre de Recherche des Cordeliers, Équipe 11 Labellisée par la Ligue Contre le Cancer, Université de Paris, Sorbonne Université, Inserm U1138, Institut Universitaire de France, Paris, France.

出版信息

Methods Cell Biol. 2021;164:137-156. doi: 10.1016/bs.mcb.2021.04.001. Epub 2021 May 1.

DOI:10.1016/bs.mcb.2021.04.001

PMID:34225912

Abstract

Fasting induces vast metabolic adaptations on the cellular level and leads to an organism-wide induction of autophagy. Autophagic degradation subserves resource recycling and facilitates the maintenance of energetic homeostasis. Mass spectrometry offers the possibility to assess changes in the metabolome that occur in conditions of nutrient deprivation and to profile such adaptations. Here we describe a detailed workflow for the targeted quantitation and untargeted profiling of metabolites that can be used to assess the intracellular metabolome of starving cells. Moreover, we outline a workflow for the use of non-radioactive isotope labeled metabolites. Altogether, we show that mass spectrometry is a powerful tool for monitoring metabolic changes in conditions of fasting.

摘要

禁食在细胞水平上诱导广泛的代谢适应，并导致全身自噬的诱导。自噬降解有助于资源回收，并有助于维持能量动态平衡。质谱法提供了评估营养剥夺条件下代谢组发生变化的可能性，并对这些适应进行分析。在这里，我们描述了一种用于定量和非靶向代谢产物分析的详细工作流程，可用于评估饥饿细胞的细胞内代谢组。此外，我们还概述了使用非放射性同位素标记代谢物的工作流程。总之，我们表明质谱法是监测禁食条件下代谢变化的有力工具。