新型荧光探针用于食品和环境样品中草甘膦的灵敏测定。

New Fluorescent Probes for the Sensitive Determination of Glyphosate in Food and Environmental Samples.

机构信息

Department of Applied Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, People's Republic of China.

出版信息

J Agric Food Chem. 2021 Nov 3;69(43):12661-12673. doi: 10.1021/acs.jafc.1c05246. Epub 2021 Oct 21.

DOI:10.1021/acs.jafc.1c05246

PMID:34672544

Abstract

In this paper, a dual-functional probe, 2-(benzothiazol)-4-(3-hydroxy-4-methylphenyl) imino phenol (), was synthesized and characterized for the simultaneous detection of Cu and Fe in dimethyl sulfoxide/4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (DMSO/HEPES) (1:4, v/v, pH = 6.0). The limits of detections (LODs) for Cu and Fe were 9.05 and 48 nM, respectively. Based on the competitive coordination, the complex exhibited good sensitivity and selectivity for glyphosate. The LODs of and for glyphosate were 0.41 and 0.63 μM, respectively. The probe quantitatively detected glyphosate in tap water, Songhua River water, local water and soil, and food samples. The colorimetric on-site glyphosate sensing through the probe was also studied based on smartphones. and exhibited outstanding imaging capabilities for Cu, Fe, and glyphosate in living cells with low cytotoxicity, especially the first time for glyphosate.

摘要

在本文中，合成并表征了一种双功能探针 2-(苯并噻唑)-4-(3-羟基-4-甲基苯基)亚氨基苯酚()，用于在二甲基亚砜/4-(2-羟乙基)-1-哌嗪乙磺酸(DMSO/HEPES)(1:4，v/v，pH=6.0)中同时检测 Cu 和 Fe。Cu 和 Fe 的检出限(LOD)分别为 9.05 和 48 nM。基于竞争性配位，配合物对草甘膦表现出良好的灵敏度和选择性。和对草甘膦的检出限分别为 0.41 和 0.63 μM。该探针定量检测了自来水中、松花江水中、当地水和土壤以及食物样品中的草甘膦。还通过智能手机研究了基于探针的草甘膦现场比色检测。和对 Cu、Fe 和草甘膦在活细胞中具有低细胞毒性的出色成像能力，尤其是首次用于草甘膦。