Long noncoding RNA promotes papillary thyroid cancer cells proliferation via regulating miR-186/CDK6 axis.

BACKGROUND

Papillary thyroid cancer (PTC) is a common endocrine malignancy, and its incidence rate has been increasing in recent years. Long noncoding RNAs (lncRNAs) participate in cell biological processes through a variety of regulatory ways, and play an essential role in tumor development.

METHODS

This study explored the expression of lncRNA small nucleolar RNA host gene 6 () in PTC by bioinformatics analysis, and quantitative real-time PCR (qRT-PCR). Cell counting kit-8 (CCK-8) assay, colony formation assay, and 5-ethynyl-2'-deoxyuridine (EdU) assay were used to study the effect of on the proliferation of PTC cells. Luciferase reporter gene assay and western blot were used to study the mechanism.

RESULTS

was highly expressed in PTC tissue samples and cell lines. , overexpression of promoted the proliferation of PTC cells, while silencing inhibited the proliferation of PTC cells. miR-186 is the downstream target of . regulates the proliferation of PTC cells through miR-186. In addition, is the target gene of miR-186, which can inhibit the expression of CDK6 protein. can promote the expression of CDK6 by regulating miR-186.

CONCLUSIONS

is highly expressed in PTC and can promote the proliferation of PTC cells by regulating the miR-186/CDK6 axis, which is expected to become a potential therapeutic target for PTC.