转录组测序鉴定与复发性自然流产相关的 ceRNA 网络。
Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion.
机构信息
Department of Reproductive Medicine, The Second Affiliated Hospital of Hainan Medical University, No. 368, Yehai Avenue, Haikou, 570311, Hainan, People's Republic of China.
出版信息
BMC Med Genomics. 2021 Nov 23;14(1):278. doi: 10.1186/s12920-021-01125-4.
BACKGROUND
Recurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families. The study aimed to explore the lncRNA-miRNA-mRNA network associated with recurrent spontaneous abortion.
METHODS
By transcriptome sequencing, we detected differences in lncRNA, miRNA and mRNA expression in villus tissue samples collected from 3 patients with RSA and 3 normal abortion patients. Differentially expressed lncRNAs, miRNAs and genes (DELs, DEMs and DEGs, respectively) were identified, and Geno Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to determine the functions of DELs and DEGs, which were analysed by Fisher's test. We also observed the regulatory relationships between miRNA-mRNA and lncRNA-miRNA by Cytoscape 3.6.1.
RESULTS
The results showed that 1008 DELs (523 upregulated and 485 downregulated), 475 DEGs (201 upregulated and 274 downregulated) and 37 DEMs (15 upregulated and 22 downregulated) were identified. And we also constructed a novel lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 genes (NTNG2, GRIA1 and AQP1).
CONCLUSIONS
lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 mRNAs (NTNG2, GRIA1 and AQP1) was constructed. The results may provide a basic theory for elucidating the mechanism underlying RSA.
背景
复发性自然流产（RSA）是妊娠的常见并发症之一，给患者及其家庭带来沉重负担。本研究旨在探讨与复发性自然流产相关的长链非编码 RNA（lncRNA）-微小 RNA（miRNA）-信使 RNA（mRNA）网络。
方法
通过转录组测序，我们检测了 3 例 RSA 患者和 3 例正常流产患者绒毛组织样本中 lncRNA、miRNA 和 mRNA 的表达差异。鉴定差异表达的 lncRNA、miRNA 和基因（分别为 DELs、DEMs 和 DEGs），并使用基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析来确定 DELs 和 DEGs 的功能，Fisher 检验进行分析。我们还通过 Cytoscape 3.6.1 观察 miRNA-mRNA 和 lncRNA-miRNA 之间的调控关系。
结果
结果显示，鉴定出 1008 个 DELs（523 个上调，485 个下调）、475 个 DEGs（201 个上调，274 个下调）和 37 个 DEMs（15 个上调，22 个下调）。我们还构建了一个包含 31 个 lncRNA、1 个 miRNA（hsa-miR-210-5p）和 3 个基因（NTNG2、GRIA1 和 AQP1）的新型 lncRNA 相关 ceRNA 网络。
结论
构建了包含 31 个 lncRNA、1 个 miRNA（hsa-miR-210-5p）和 3 个 mRNA（NTNG2、GRIA1 和 AQP1）的 lncRNA 相关 ceRNA 网络。研究结果可为阐明 RSA 发病机制提供基础理论。
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