基于罗丹明衍生物的近红外荧光探针用于活细胞中 NADH 的检测。
Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells.
机构信息
Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA.
Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA.
出版信息
Methods. 2022 Aug;204:22-28. doi: 10.1016/j.ymeth.2022.03.019. Epub 2022 Apr 2.
Abstract
A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction.
摘要
制备了一种近红外荧光探针，用于选择性检测活细胞中的还原型烟酰胺腺嘌呤二核苷酸（NADH）。探针通过闭合螺内酯开关使荧光关闭。然而，NADH 还原探针使其在 740nm 处产生荧光。理论计算表明，还原后，罗丹明和喹啉部分之间的平面排列更规则，π 离域增强。
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