一种用于在水性介质和活细胞中级联传感铝离子和氟离子的灵敏的开-关-开荧光探针。
A sensitive OFF-ON-OFF fluorescent probe for the cascade sensing of Al and F ions in aqueous media and living cells.
作者信息
Hou Lingjie, Liang Wenting, Deng Chenhua, Zhang Caifeng, Liu Bo, Shuang Shaomin, Wang Yu
机构信息
Department of Chemistry, Taiyuan Normal University Jinzhong 030619 P. R. China.
Humic Acid Engineering and Technology Research Center of Shanxi Province Jinzhong 030619 P. R. China.
出版信息
RSC Adv. 2020 Jun 5;10(36):21629-21635. doi: 10.1039/d0ra02848g. eCollection 2020 Jun 2.
A simple Schiff-base ligand 2-hydroxy-1-naphthaldehyde semicarbazone (HNS) was synthesized and characterized. Based on the combined effect of inhibition of CH[double bond, length as m-dash]N isomerization and chelation-enhanced fluorescence (CHEF), HNS functions as a fluorescence "turn on" sensor for Al in buffered aqueous media. Based on the strong affinity of Al to F ions, the generated Al-HNS complex can also be utilized as an effective chemosensor for F sensing by metal displacement approach, ensuing quenching of fluorescence by the reversible return of HNS from Al-HNS complex. Thus a method using a single probe for the detection of both Al and F ions is developed. The system exhibits high selectivity and sensitivity for Al and F ions and the detection limits were found to be as low as 6.75 × 10 M and 7.89 × 10 M, respectively. Furthermore, the practical applicability of this probe has been examined in living cells.
合成并表征了一种简单的席夫碱配体2-羟基-1-萘甲醛缩氨基脲（HNS）。基于抑制C=N异构化和螯合增强荧光（CHEF）的综合作用，HNS在缓冲水性介质中作为铝的荧光“开启”传感器发挥作用。基于铝对氟离子的强亲和力，生成的铝-HNS络合物也可通过金属置换法用作检测氟的有效化学传感器，通过HNS从铝-HNS络合物中可逆返回导致荧光猝灭。因此，开发了一种使用单一探针检测铝和氟离子的方法。该系统对铝和氟离子具有高选择性和灵敏度，检测限分别低至6.75×10⁻⁸ M和7.89×10⁻⁸ M。此外，还在活细胞中检验了该探针的实际适用性。
Zotero 插件