Investigation on characterization, antifouling and cytotoxic properties of zinc oxide nanoparticles biosynthesized by a mangrove-associated actinobacterium Streptomyces olivaceus (MSU3).

The present study was undertaken to biosynthesize zinc oxide nanoparticles (ZnONPs) using a mangrove-associated actinobacterium Streptomyces olivaceus (MSU3) under in vitro conditions. The synthesized ZnONPs were structurally characterized through UV, FT-IR, TG-DTA, XRD, SEM and EDX analysis. Analysis of biosynthesized ZnONPs in UV-Vis spectroscopy showed presence of functional groups between the wavelengths 325 and 380 nm. FT-IR analysis showed the functional groups, such as halo bromide (C-Br), alkyne (C≡C), carboxylic acid (O-H), nitro (N-O), fluoro (C-F), alkene (C=C) and aromatic (R-C-H) groups, respectively, within the wave numbers between 614.30 and 3074.41 cm. The crystalline poly-dispersed quasi spherical nature of ZnONPs expressed the average particle size of 37.9 nm with the 2θ values of 11.802-37.885°. Antibacterial activity of ZnONPs showed pronounced inhibitory zone (25 mm) and least MIC and MBC values (125 and 250 µg ml) against Escherchia sp. In the antifouling study, ZnONPs strongly inhibited byssal thread formation in mussel Perna indica and recorded LC value of 424.47 µg ml. Mollusc foot adherence assay inferred that the ZnONPs effectively inhibited settlement of limpet Patella vulgata and showed minimal fouling (26.43%) at 350 µg ml and recorded LC value of 218.77 µg ml. Results of anticrustacean assay depicted that, ZnONPs had registered LC value of 676.08 µg ml against Artemia salina nauplii. From this study, it could be concluded that an eco-friendly approach could be used to open a new avenue for biosynthesis of ZnONPs from a mangrove associated actinobacterium S. olivaceus (MSU3) in antifouling studies.