膳食黄曲霉毒素 B 导致生长抑制，并激活氧化应激和内质网应激途径，诱导北方蛇头鱼（Channa argus）肝脏凋亡和炎症。
Dietary aflatoxin B caused the growth inhibition, and activated oxidative stress and endoplasmic reticulum stress pathway, inducing apoptosis and inflammation in the liver of northern snakehead (Channa argus).
机构信息
College of Animal Science and Technology, Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Key Laboratory of Animal Production, Product Quality and Security, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun 130118, China.
College of Animal Science and Technology, Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Key Laboratory of Animal Production, Product Quality and Security, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun 130118, China.
出版信息
Sci Total Environ. 2022 Dec 1;850:157997. doi: 10.1016/j.scitotenv.2022.157997. Epub 2022 Aug 12.
The purpose of this study was to investigate the effects of dietary aflatoxin B (AFB) on growth performance and AFB biotransformation, and hepatic oxidative stress, endoplasmic reticulum (ER) stress, apoptosis, and inflammation in northern snakehead (Channa argus). A total of 600 northern snakeheads (7.52 ± 0.02 g) were divided into five groups (three replicates/group) and fed the diets with AFB at concentrations of 0, 50, 100, 200, and 400 ppb for 8 weeks. The results demonstrated that dietary AFB (≥ 200 ppb) reduced FBW, WG, and SGR. 100, 200, and 400 ppb AFB treatment groups significantly decreased the PER, CRP, C3, C4, IgM, and LYS levels in northern snakehead, while FCR was significant increased. Moreover, dietary AFB (100, 200, and 400 ppb) increased cyp1a, cyp1b (except 400 ppb), and cyp3a mRNA expression levels, while reducing the GST enzymatic activity and mRNA expression levels in northern snakehead. Furthermore, AFB (≥ 100 ppb) increased ROS, MDA, and 8-OHdG levels, and grp78, ire1, perk, jnk, chop, and traf2 mRNA expression levels, and decreased SOD, CAT, GSH-Px, and GSH (except 100 ppb) levels and the gene expression levels of cat, gsh-px (except 100 ppb), and Cu/Zn sod. In addition, AFB (100, 200, and 400 ppb) up-regulated the cyt-c, bax, cas-3, and cas-9 mRNA levels in the liver, while down-regulating the bcl-2 expression levels. Meanwhile, the expression levels of nf-κb, tnf-α (except 100 ppb), il-1β, and il-8 in the liver were up-regulated in AFB treatment groups (≥ 100 ppb), while the iκbα mRNA levels were down-regulated. In summary, dietary AFB reduced growth performance and humoral immunity in northern snakehead. Meanwhile, the cyclic occurrence of oxidative stress and ER stress, and induced apoptosis and inflammation, is one of the main reasons for AFB-induced liver injury in the northern snakehead, which will provide valuable information and a fresh perspective for further research into AFB-induced liver injury in fish.
本研究旨在探究黄曲霉毒素 B（AFB）对北方蛇头鱼生长性能和 AFB 生物转化、肝脏氧化应激、内质网应激、细胞凋亡和炎症的影响。将 600 条北方蛇头鱼（7.52±0.02g）分为 5 组（每组 3 个重复），用浓度为 0、50、100、200 和 400ppb 的 AFB 饲料喂养 8 周。结果表明，膳食 AFB（≥200ppb）降低了 FBW、WG 和 SGR。100、200 和 400ppb AFB 处理组显著降低了北方蛇头鱼的 PER、CRP、C3、C4、IgM 和 LYS 水平，而 FCR 显著升高。此外，膳食 AFB（100、200 和 400ppb）增加了 cyp1a、cyp1b（400ppb 除外）和 cyp3a 的 mRNA 表达水平，同时降低了 GST 酶活性和北方蛇头鱼的 mRNA 表达水平。此外，AFB（≥100ppb）增加了 ROS、MDA 和 8-OHdG 水平，grp78、ire1、perk、jnk、chop 和 traf2 的 mRNA 表达水平，并降低了 SOD、CAT、GSH-Px 和 GSH（100ppb 除外）水平以及 cat、gsh-px（100ppb 除外）和 Cu/Zn sod 的基因表达水平。此外，AFB（100、200 和 400ppb）上调了肝脏中 cyt-c、bax、cas-3 和 cas-9 的 mRNA 水平，同时下调了 bcl-2 的表达水平。同时，NF-κB、TNF-α（100ppb 除外）、IL-1β和 IL-8 在肝脏中的表达水平在 AFB 处理组（≥100ppb）中上调，而 IκBα的 mRNA 水平下调。总之，膳食 AFB 降低了北方蛇头鱼的生长性能和体液免疫。同时，氧化应激和内质网应激的周期性发生，以及诱导的细胞凋亡和炎症，是 AFB 引起北方蛇头鱼肝损伤的主要原因之一，这将为进一步研究鱼类 AFB 引起的肝损伤提供有价值的信息和新视角。
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