猫冠状病毒变异体在非猫传染性腹膜炎猫中的检测。
Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis.
机构信息
Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, Veterinärstraße 13, 80539 Munich, Germany.
Section of Clinical and Comparative Neuropathology, Institute of Veterinary Pathology, Centre for Clinical Veterinary Medicine, LMU Munich, Veterinärstraße 13, 80539 Munich, Germany.
出版信息
Viruses. 2022 Jul 29;14(8):1671. doi: 10.3390/v14081671.
(1) Background: This study aimed to detect feline coronavirus (FCoV) and characterize spike (S) gene mutation profiles in cats suffering from diseases other than feline infectious peritonitis (FIP) using commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) and reevaluating results by sequencing. (2) Methods: In 87 cats in which FIP was excluded by histopathology and immunohistochemistry, FCoV 7b gene and S gene mutation RT-qPCR was performed prospectively on incisional biopsies and fine-needle aspirates of different organs, body fluids, and feces. Samples positive for S gene mutations or mixed FCoV underwent sequencing. (3) Results: In 21/87 cats, FCoV RNA was detectable. S gene mutations were detected by commercial RT-qPCR (and a diagnostic algorithm that was used at the time of sample submission) in at least one sample in 14/21 cats (66.7%), with only mutated FCoV in 2/21, only mixed in 1/21, and different results in 11/21 cats; in the remaining 7/21 cats, RNA load was too low to differentiate. However, sequencing of 8 tissue samples and 8 fecal samples of 9 cats did not confirm mutated FCoV in any of the FCoV RNA-positive cats without FIP. (4) Conclusions: Sequencing results did not confirm results of the commercial S gene mutation RT-qPCR.
(1) 背景：本研究旨在使用商业实时逆转录聚合酶链反应（RT-qPCR）检测患有除猫传染性腹膜炎（FIP）以外疾病的猫中猫冠状病毒（FCoV），并通过测序对结果进行重新评估，以检测和分析冠状病毒 Spike（S）基因的突变特征。(2) 方法：在 87 例经组织病理学和免疫组织化学排除 FIP 的猫中，对不同器官、体液和粪便的切取活检和细针抽吸物进行 FCoV 7b 基因和 S 基因突变 RT-qPCR，对 S 基因发生突变或混合感染的 FCoV 样本进行测序。(3) 结果：在 87 例猫中，有 21 例可检测到 FCoV RNA。在 21 例猫中，有 14 例（66.7%）至少有一个样本通过商业 RT-qPCR（以及当时提交样本时使用的诊断算法）检测到 S 基因发生突变，但在 21 例猫中只有 2 例只有突变 FCoV，1 例只有混合 FCoV，11 例结果不同；在其余 7 例猫中，由于 RNA 载量太低而无法区分。然而，对 9 只猫的 8 个组织样本和 8 个粪便样本进行测序，在没有 FIP 的 FCoV RNA 阳性猫中，未确认任何猫存在突变 FCoV。(4) 结论：测序结果无法证实商业 S 基因突变 RT-qPCR 的结果。
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