CDCA3 在促进卵巢癌进展中发挥作用。
CDCA3 exhibits a role in promoting the progression of ovarian cancer.
机构信息
Department of Gynecology, The Fourth Hospital of Hebei Medical University, No. 12 Jiankang Road, Shijiazhuang 050011, China; Department of Gynecology, The First Hospital of Qinhuangdao, No. 252 Wenhua Road, Qinhuangdao 066000, China.
Department of Orthopedics, The First Hospital of Qinhuangdao, Qinhuangdao, Hebei Province 066000, China.
出版信息
Tissue Cell. 2022 Dec;79:101903. doi: 10.1016/j.tice.2022.101903. Epub 2022 Aug 24.
OBJECTIVE
Ovarian cancer (OC) is one of the common gynecological malignant tumors. Cell division cycle-associated protein-3 (CDCA3) is involved in the regulation of cell cycle progression. The role of CDCA3 in OC was explored in this study.
METHODS
The expression of CDCA3 in OC was evaluated in the Gene Expression Omnibus (GEO) database and further verified by qRT-PCR and Western blot (WB). Subsequently, we established lentivirus-mediated CDCA3 knockdown in OC cell lines HO-8910 and A2780. The biological roles of CDCA3 on proliferation, sensitivity to cisplatin, apoptosis, migration and tumor formation of OC were investigated using loss-of-function assays.
RESULTS
CDCA3 was frequently up-regulated in OC. Knockdown of CDAC3 inhibited proliferation and migration, and enhanced apoptosis as well as sensitivity of OC cells to cisplatin. In vivo results further confirmed the inhibitory effect of CDCA3 knockdown on tumor growth.
CONCLUSIONS
Our findings indicated that CDCA3 may play an important role in OC progression, and may serve as a potential therapeutic target for the OC treatment.
目的
卵巢癌（OC）是常见的妇科恶性肿瘤之一。细胞分裂周期相关蛋白 3（CDCA3）参与细胞周期进程的调节。本研究探讨了 CDCA3 在 OC 中的作用。
方法
在基因表达综合数据库（GEO）中评估 CDCA3 在 OC 中的表达，并通过 qRT-PCR 和 Western blot（WB）进一步验证。随后，我们在 OC 细胞系 HO-8910 和 A2780 中建立了慢病毒介导的 CDCA3 敲低。通过功能丧失试验研究了 CDCA3 对 OC 细胞增殖、顺铂敏感性、细胞凋亡、迁移和肿瘤形成的生物学作用。
结果
CDCA3 在 OC 中频繁上调。CDAC3 敲低抑制了 OC 细胞的增殖和迁移，并增强了细胞凋亡和对顺铂的敏感性。体内结果进一步证实了 CDCA3 敲低对肿瘤生长的抑制作用。
结论
我们的研究结果表明，CDCA3 可能在 OC 进展中发挥重要作用，并可能成为 OC 治疗的潜在治疗靶点。
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