细胞分裂周期相关蛋白 3 在前列腺癌中的预后价值。
Prognostic value of cell division cycle-associated protein-3 in prostate cancer.
机构信息
Department of Urology, Xishan People's Hospital of Wuxi City, Wuxi, Jiangsu, China.
Department of Operating Room, Xishan People's Hospital of Wuxi City, Wuxi, Jiangsu, China.
出版信息
Medicine (Baltimore). 2023 Sep 8;102(36):e34655. doi: 10.1097/MD.0000000000034655.
BACKGROUND
The study was aimed to explore cell division cycle-associated protein-3 (CDCA3) expression and its correlation with clinicopathological characteristics, and identification of co-expressed genes of CDCA3 in prostate cancer (PCa).
METHODS
Data for CDCA3 mRNA expression in PCa were obtained from The Cancer Genome Atlas database. Furtherly, CDCA3 protein expression was examined by immunohistochemistry in 80 cases, including 20 normal prostate samples and 60 PCa samples. Then we used "survival" package to obtain the differentially expressed CDCA3 mRNA associated with prognosis of PCa patients. "pROC" package was used to analyze receiver operating characteristic of CDCA3. We used chi-square test, Kruskal-Wallis test and Wilcoxon rank sum test to identify clinicopathological parameters that correlated with CDCA3 expression. In order to determine the effects of CDCA3 expression and clinicopathological parameters on survival, univariate cox regression analysis was performed. Finally, the co-expressed genes of CDCA3 in PCa were explored by search tool for the retrieval of interacting genes, Kyoto encyclopedia of genes and genomes enrichment analysis and Spearman correlation analysis.
RESULTS
In this study, we found that CDCA3 expression was increased in PCa. PCa patients with higher CDCA3 expression had poor outcomes. In terms of receiver operating characteristic, CDCA3 had an area under the curve of 0.857. High CDCA3 expression was positively correlated with advanced T stage, N stage, Gleason score, and served as an independent predictor of progress free interval in PCa patients. Then 20 proteins closely related to CDCA3 were screened through STRING website. Functional enrichment analysis revealed that, Kyoto encyclopedia of genes and genomes pathway was mainly enriched in cell cycle, including 6 genes, BUB1, CCNA2, CDK1, CDC20, TTK, and CCNB2.
CONCLUSION
CDCA3 is significantly associated with the prognosis of PCa, which may be an indicator of the diagnosis and prognosis of PCa and a new therapeutic target.
背景
本研究旨在探讨细胞分裂周期相关蛋白 3（CDCA3）的表达及其与前列腺癌（PCa）临床病理特征的相关性，并鉴定 CDCA3 在 PCa 中的共表达基因。
方法
从癌症基因组图谱数据库中获取 CDCA3 mRNA 在 PCa 中的表达数据。进一步，通过免疫组织化学法检测 80 例样本中 CDCA3 蛋白的表达，包括 20 例正常前列腺样本和 60 例 PCa 样本。然后我们使用“survival”包获取与 PCa 患者预后相关的差异表达的 CDCA3 mRNA。使用“pROC”包分析 CDCA3 的受试者工作特征。我们使用卡方检验、Kruskal-Wallis 检验和 Wilcoxon 秩和检验来确定与 CDCA3 表达相关的临床病理参数。为了确定 CDCA3 表达和临床病理参数对生存的影响，我们进行了单因素 Cox 回归分析。最后，通过搜索工具 for the retrieval of interacting genes、京都基因与基因组百科全书富集分析和 Spearman 相关分析，探索 PCa 中 CDCA3 的共表达基因。
结果
在本研究中，我们发现 CDCA3 在 PCa 中表达增加。CDCA3 表达较高的 PCa 患者预后较差。在受试者工作特征方面，CDCA3 的曲线下面积为 0.857。高 CDCA3 表达与晚期 T 分期、N 分期、Gleason 评分呈正相关，是 PCa 患者无进展间隔的独立预测因子。然后通过 STRING 网站筛选出与 CDCA3 密切相关的 20 种蛋白质。功能富集分析表明，京都基因与基因组百科全书通路主要富集在细胞周期中，包括 6 个基因，BUB1、CCNA2、CDK1、CDC20、TTK 和 CCNB2。
结论
CDCA3 与 PCa 的预后显著相关，可能是 PCa 诊断和预后的指标，也是新的治疗靶点。
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