在单细胞和单囊泡分辨率下监测质膜损伤触发的内吞作用。
Monitoring Plasma Membrane Injury-Triggered Endocytosis at Single-Cell and Single-Vesicle Resolution.
机构信息
Center for Genetic Medicine Research, Children's National Research Institute, Washington, DC, USA.
Department of Genomics and Precision Medicine, George Washington University School of Medicine and Health Sciences, Washington, DC, USA.
出版信息
Methods Mol Biol. 2023;2587:513-526. doi: 10.1007/978-1-0716-2772-3_27.
Plasma membrane injury activates membrane trafficking and remodeling events that are required for the injured membrane to repair. With the rapidity of the membrane repair process, the repair response needs to be monitored at high temporal and spatial resolution. In this chapter, we describe the use of live cell optical imaging approaches to monitor injury-triggered bulk and individual vesicle endocytosis. Use of these approaches allows quantitatively assessment of the rate of retrieval of the injured plasma membrane by bulk endocytosis as well as by endocytosis of individual caveolae following plasma membrane injury.
质膜损伤会激活膜运输和重塑事件，这些事件是受伤膜修复所必需的。由于膜修复过程非常迅速，因此需要以高时间和空间分辨率来监测修复反应。在本章中，我们描述了使用活细胞光学成像方法来监测损伤触发的胞吞作用。这些方法的使用可以定量评估质膜损伤后通过胞吞作用整体回收受伤质膜的速度以及通过单个 caveolae 的内吞作用回收受伤质膜的速度。
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