一步法合成具有可调谐荧光和增强基因转染效率的肽-金纳米簇。
One-Step Synthesis of Peptide-Gold Nanoclusters with Tunable Fluorescence and Enhanced Gene Delivery Efficiency.
机构信息
State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P. R. China.
Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, P. R. China.
出版信息
Langmuir. 2022 Dec 6;38(48):14799-14807. doi: 10.1021/acs.langmuir.2c02465. Epub 2022 Nov 21.
In this study, peptide-gold nanoclusters with tunable fluorescence were prepared by a simple "one-pot" method, which were used for gene localization and delivery in vivo to achieve efficient intracellular colocalization, uptake, and transfection. The efficiency of pDNA transfection was up to 70.6%, and there was no obvious cytotoxicity. This study proves that the simple-composition and bio-friendly peptide-gold nanoclusters are promising gene delivery carriers and can provide a powerful theoretical and experimental basis for the application of peptide-metal nanocomplexes in gene delivery and other biomedicine fields.
在这项研究中，通过一种简单的“一锅法”制备了具有可调荧光的肽-金纳米簇，用于体内基因定位和递送，以实现高效的细胞内共定位、摄取和转染。pDNA 的转染效率高达 70.6%，且没有明显的细胞毒性。本研究证明了组成简单、生物友好的肽-金纳米簇是很有前途的基因递送载体，并可为肽-金属纳米复合物在基因递送和其他生物医学领域的应用提供强有力的理论和实验基础。
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