马远跖趾关节骨关节炎的 microRNA 生物标志物研究。

Investigation of MicroRNA Biomarkers in Equine Distal Interphalangeal Joint Osteoarthritis.

机构信息

The Roslin Institute, The University of Edinburgh, Easter Bush, Roslin EH25 9RG, UK.

The RICE Group, Division of Gene Function and Development, Roslin Institute, The University of Edinburgh, Easter Bush, Roslin EH25 9RG, UK.

出版信息

Int J Mol Sci. 2022 Dec 8;23(24):15526. doi: 10.3390/ijms232415526.

DOI:10.3390/ijms232415526

PMID:36555166

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9779011/

Abstract

Osteoarthritis of the equine distal interphalangeal joint is a common cause of lameness. MicroRNAs from biofluids are promising biomarkers and therapeutic candidates. Synovial fluid samples from horses with mild and severe equine distal interphalangeal joint osteoarthritis were submitted for small RNA sequencing. The results demonstrated that miR-92a was downregulated in equine synovial fluid from horses with severe osteoarthritis and there was a significant increase in COMP, COL1A2, RUNX2 and SOX9 following miR-92a mimic treatment of equine chondrocytes in monolayer culture. This is the first equine study to evaluate the role of miR-92a in osteoarthritic chondrocytes in vitro.

摘要

马的远指关节骨关节炎是跛行的常见原因。生物流体中的 microRNAs 是很有前途的生物标志物和治疗候选物。轻度和重度马远指关节骨关节炎的马的滑液样本被提交进行小 RNA 测序。结果表明，miR-92a 在严重骨关节炎马的滑液中下调，并且在单层培养的马软骨细胞中用 miR-92a 模拟物处理后，COMP、COL1A2、RUNX2 和 SOX9 显著增加。这是第一项评估 miR-92a 在体外骨关节炎软骨细胞中的作用的马研究。