Yu Yao, Wang Ziyao, Xu Yiling, Ma Bojun, Chen Xifeng
College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Feb 25;39(2):724-740. doi: 10.13345/j.cjb.220578.
gene is a group of key genes regulating plant growth and development. Here, gene families of strawberry were identified from the genome of the diploid , and their physicochemical properties, genes structure, evolution and genes expression were also analyzed. Our results showed that there were thirty-one genes in . and the encoded proteins were classified into seven groups, and the members in the same group showed high similarity in gene structures and conservative motifs. The electronic subcellular localization of FvSUNs was mainly in the nucleus. Collinearity analysis showed that the members of gene family were mainly expanded by segmental duplication in . , and and . shared twenty-three pairs of orthologous genes. According to the expression pattern in different tissues shown by the transcriptome data of . , the gene can be divided into three types: (1) expressed in nearly all tissues, (2) hardly expressed in any tissues, and (3) expressed in special tissues. The gene expression pattern of was further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, the seedlings of . were treated by different abiotic stresses, and the expression level of 31 genes were assayed by qRT-PCR. The expression of most of the tested genes was induced by cold, high salt or drought stress. Our studies may facilitate revealing the biological function and molecular mechanism of genes in strawberry.
基因是一组调控植物生长发育的关键基因。在此,从二倍体草莓的基因组中鉴定出草莓的基因家族,并对其理化性质、基因结构、进化和基因表达进行了分析。我们的结果表明,草莓中有31个基因,这些基因编码的蛋白质被分为七组,同一组中的成员在基因结构和保守基序上表现出高度相似性。FvSUNs的电子亚细胞定位主要在细胞核中。共线性分析表明,草莓基因家族的成员主要通过片段重复进行扩增,且草莓与其他物种共享23对直系同源基因。根据草莓转录组数据显示的不同组织中的表达模式,该基因可分为三种类型:(1)在几乎所有组织中表达;(2)在任何组织中几乎不表达;(3)在特殊组织中表达。通过实时定量聚合酶链反应(qRT-PCR)进一步验证了该基因的表达模式。此外,对草莓幼苗进行不同非生物胁迫处理,并通过qRT-PCR检测31个基因的表达水平。大多数测试基因的表达受冷、高盐或干旱胁迫诱导。我们的研究可能有助于揭示草莓中该基因的生物学功能和分子机制。