刺山柑（十字花科）中硫代葡萄糖苷馏分的抗利什曼原虫活性

Anti-Leishmanial Activity of Glucosinolate Fraction from Steph. ex Willd (Brassicaceae).

作者信息

Mirzaee Fatemeh, Faridnia Roghiyeh, Fakhar Mahdi, Kalani Hamed, Shahani Somayeh

机构信息

Mazandaran University of Medical Sciences, Medicinal Plants Research Center, Sari, Iran.

Golestan University of Medical Sciences, Laboratory Sciences Research Center, Gorgan, Iran.

出版信息

Turk J Pharm Sci. 2023 Mar 2;20(1):16-22. doi: 10.4274/tjps.galenos.2022.78027.

DOI:10.4274/tjps.galenos.2022.78027

PMID:36862022

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9986943/

Abstract

OBJECTIVES

The intracellular parasitic protozoan, spp., causes several forms of diseases in humans. Cytotoxicity and emergence of new strains resistance to the current anti-leishmanial drugs have encouraged researchers to focus on new resources. Glucosinolates (GSL) are found mainly in the Brassicaceae family with potential cytotoxic and anti-parasitic properties. The present study reports antileishmanial activity of the GSL fraction from seeds against .

MATERIALS AND METHODS

The GSL fraction was prepared by ion-exchange and reversed-phase chromatography. For the assessment of antileishmanial activity, the promastigotes and amastigotes of were treated with different concentrations of the fraction (75-625 μg/mL).

RESULTS

The IC was 245 µg/mL for anti-promastigote effect of the GSL fraction and 250 µg/mL for its anti-amastigote effect that had a significant difference (<0.05) with both glucantime and amphotericin B. The selectivity index of the GSL fraction (15.8), to glucantime and amphotericin B, was greater than 10, indicating the selective effect of this fraction against amastigotes. Glucoiberverin was the major constituent of the GSL fraction characterized using nuclear magnetic resonance and electron ionization-mass spectrometry spectra. Based on gas chromatography-mass spectrometry data, iberverin and iberverin nitrile, the hydrolysis constituents from glucoiberverin, included 76.91% of the total seed volatiles.

CONCLUSION

The results suggest that GSLs like glucoiberverin could be considered a new promising candidate for further studies on antileishmanial activity.

摘要

目的

细胞内寄生原生动物利什曼原虫属的某些物种可引发人类多种疾病。细胞毒性以及对当前抗利什曼原虫药物产生耐药性的新菌株的出现，促使研究人员将重点放在新资源上。硫代葡萄糖苷（GSL）主要存在于十字花科植物中，具有潜在的细胞毒性和抗寄生虫特性。本研究报告了来自[种子名称]种子的GSL组分对[利什曼原虫名称]的抗利什曼原虫活性。

材料与方法

通过离子交换和反相色谱法制备GSL组分。为评估抗利什曼原虫活性，用不同浓度的该组分（75 - 625μg/mL）处理[利什曼原虫名称]的前鞭毛体和无鞭毛体。

结果

GSL组分抗前鞭毛体作用的半数抑制浓度（IC）为245μg/mL，抗无鞭毛体作用的IC为250μg/mL，与葡糖胺锑钠和两性霉素B相比均有显著差异（<0.05）。GSL组分对葡糖胺锑钠和两性霉素B的选择性指数（15.8）大于10，表明该组分对[利什曼原虫名称]无鞭毛体具有选择性作用。葡糖异硫氰酸烯丙酯是通过核磁共振和电子电离质谱光谱鉴定的GSL组分的主要成分。基于气相色谱 - 质谱数据，异硫氰酸烯丙酯和异硫氰酸烯丙酯腈这两种葡糖异硫氰酸烯丙酯的水解成分占种子挥发物总量的76.91%。