一种新型的基于去氢枞酸的多功能荧光探针，用于 Cu/Zn/ClO 的检测和生物成像。
A novel dehydroabietic acid-based multifunctional fluorescent probe for the detection and bioimaging of Cu/Zn/ClO.
机构信息
Jiangsu Provincial Key Lab for the Chemistry and Utilization of Agro-forest Biomass, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, P. R. China.
出版信息
Analyst. 2023 Apr 11;148(8):1867-1876. doi: 10.1039/d3an00001j.
A multifunctional dehydroabietic acid-based fluorescent probe (CPS) was designed and synthesized by introducing the 2,6-bis(1-benzo[]imidazol-2-yl)phenol fluorophore. The probe CPS could selectively recognize Cu, Zn and ClO ions from other analytes, and it showed fluorescence quenching behavior toward Cu and a ratiometric response to Zn and ClO by changing from green fluorescence to blue and cyan, respectively. The detection limits toward Cu, Zn and ClO ions were 3.8 nM, 0.253 μM and 0.452 μM, respectively. In addition, CPS presented many fascinating merits, such as high selectivity, a short response time (15-20 s), a wide pH range (3-10) and high photostability. The sensing mechanisms of CPS were verified by H-NMR, ESI-MS, FT-IR and Job's plot methods. Meanwhile, CPS exhibited satisfactory detection performance in water samples. More importantly, the probe could be applied as a promising tool for visual bioimaging of three ions in living cells and zebrafishes.
一种多功能枞酸基荧光探针（CPS）被设计和合成，通过引入 2,6-双（1-苯并[]咪唑-2-基）苯酚荧光团。探针 CPS 可以选择性地识别 Cu、Zn 和 ClO 离子与其他分析物，并且它对 Cu 表现出荧光猝灭行为，对 Zn 和 ClO 则分别表现出比率响应，从绿色荧光转变为蓝色和青色。对 Cu、Zn 和 ClO 离子的检测限分别为 3.8 nM、0.253 μM 和 0.452 μM。此外，CPS 具有许多引人注目的优点，如高选择性、短响应时间（15-20 秒）、宽 pH 范围（3-10）和高光稳定性。通过 H-NMR、ESI-MS、FT-IR 和 Job 图方法验证了 CPS 的传感机制。同时，CPS 在水样中表现出令人满意的检测性能。更重要的是，该探针可作为一种有前途的工具，用于在活细胞和斑马鱼中可视化检测三种离子。
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