使用 Truenat MTB-Rif Dx 和 GeneXpert MTB/RIF 快速诊断儿童结核病。
Expedited diagnosis of pediatric tuberculosis using Truenat MTB-Rif Dx and GeneXpert MTB/RIF.
机构信息
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India.
Division of Epidemiology and Communicable Diseases, Indian Council of Medical Research, New Delhi, India.
出版信息
Sci Rep. 2023 Apr 28;13(1):6976. doi: 10.1038/s41598-023-32810-2.
Rapid, cost-effective, and sensitive diagnostic assays are essential for global tuberculosis (TB) control, especially in high TB burden, resource-limited settings. The current study was designed to evaluate diagnostic accuracy of Truenat MTB-Rif Dx (MolBio) in children less than 18 years of age, with symptoms suggestive of TB. Gastric aspirate, induced sputum, and broncho-alveolar lavage samples were subjected simultaneously to AFB-smear, GeneXpert MTB/RIF, liquid culture (MGIT-960) and Truenat MTB-Rif Dx. The index-test results were evaluated against microbiological reference standards (MRS). Truenat MTB-Rif Dx had a sensitivity of 57.1%, specificity of 92% against MRS. The sensitivity and specificity of the Truenat MTB-RIF Dx compared with liquid culture was 58.7% and 87.5% while GeneXpert MTB/RIF was 56% and 91.4%. The performance of both GeneXpert MTB/RIF and Truenat MTB-Rif Dx are comparable. Result of our study demonstrates that Truenat MTB-Rif can aid in early and efficient diagnosis of TB in children.
快速、经济、敏感的诊断检测对于全球结核病（TB）控制至关重要，特别是在高结核病负担、资源有限的环境中。本研究旨在评估 Truenat MTB-Rif Dx（MolBio）在有结核病症状的 18 岁以下儿童中的诊断准确性。同时对胃抽吸物、诱导痰和支气管肺泡灌洗液样本进行 AFB 涂片、GeneXpert MTB/RIF、液体培养（MGIT-960）和 Truenat MTB-Rif Dx 检测。将指标检测结果与微生物学参考标准（MRS）进行比较。Truenat MTB-Rif Dx 对 MRS 的敏感性为 57.1%，特异性为 92%。Truenat MTB-RIF Dx 与液体培养相比的敏感性和特异性分别为 58.7%和 87.5%，而 GeneXpert MTB/RIF 分别为 56%和 91.4%。GeneXpert MTB/RIF 和 Truenat MTB-Rif Dx 的性能相当。我们的研究结果表明，Truenat MTB-Rif 可以帮助儿童早期和有效地诊断结核病。
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