检测中欧伏翼蝙蝠中 、 和 spp. 的分子特征。
Molecular Detection of , and spp. in Vespertilionid Bats from Central Europe.
机构信息
Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 61242 Brno, Czech Republic.
Department of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 61242 Brno, Czech Republic.
出版信息
Int J Mol Sci. 2023 Jun 8;24(12):9887. doi: 10.3390/ijms24129887.
Bats may carry various viruses and bacteria which can be harmful to humans, but little is known about their role as a parasitic source with zoonotic potential. The aim of this study was to test wild bats for the presence of selected parasites: , and microsporidia spp. In total, brain and small intestine tissues of 100 bats (52 , 43 and 5 ) were used for the DNA isolation and PCR detection of the abovementioned agents. DNA was detected by real-time PCR in 1% of bats (in one male of ), while all bats were negative for DNA. spp. DNA was detected by nested PCR in 25% of bats, including three species (twenty-two , two and one ). Positive samples were sequenced and showed homology with the genotypes II and 2C. This is the first study on wild vespertilionid bats from Central Europe and worldwide, with a relatively high positivity of spp. detected in bats.
蝙蝠可能携带各种对人类有害的病毒和细菌，但人们对它们作为具有人畜共患潜力的寄生虫源的作用知之甚少。本研究的目的是检测野生蝙蝠中是否存在选定的寄生虫：原生动物、球虫和微孢子虫属。总共使用了 100 只蝙蝠（52 只、43 只和 5 只）的脑组织和小肠组织进行上述试剂的 DNA 分离和 PCR 检测。在 1%的蝙蝠（1 只雄性中）中检测到 DNA，而所有蝙蝠的 DNA 均为阴性。通过巢式 PCR 在 25%的蝙蝠中检测到 属 DNA，包括三个物种（22 只、2 只和 1 只）。阳性样本进行测序，与基因型 II 和 2C 具有同源性。这是中欧和全球首次对野生蝙蝠进行的研究，检测到蝙蝠中 属的阳性率相对较高。
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