In vitro and in vivo antileishmanial effects of extract against .

Due to the increasing resistance to common medicinal compounds, the use of medicinal plants has received special attention. Therefore, the current survey was designed to study the antileishmanial effects of Trautv. methanolic extract against In this study, after preparing the methanolic extract of , its effect on the amastigotes of and triggering the nitric oxide (NO) were measured. Then, the in vivo effect of the methanol extract on cutaneous leishmaniasis in mice was evaluated. The best anti-amastigote effect was for the methanol extract of along with meglumine antimony with 50% inhibitory concentrations value of 17.4 μg/ml ( < 0.001). The 50% cytotoxic concentrations values of methanol extract, meglumine antimoniate, and methanol extract + meglumine antimoniate were 596.3, 784.6, and 296.4 µg/ml, respectively. Macrophages treated with the methanolic extract markedly ( < 0.001) induced the release of nitric oxide. After 28 days of treatment, lesions were completely ( < 0.001) healed in mice treated with the methanolic extract (100 mg/kg) + meglumine antimoniate (25 mg/kg). methanolic extract mainly in combination with meglumine antimoniate showed favorable antileishmanial effects on , concluding that the methanolic extract of can be used for the production of new leishmanicidal agents agaist cutaneous leishmaniasis. Although we revealed that NO trigerring and inhibition of infection in host cells are the antileishmanial mechanism action of methanolic extract, more studies must be performed to clear the mechanisms and its safety.