Regulates the Proliferation and Differentiation of C2C12 Myoblasts by Targeting BTG2.

Skeletal muscle, a vital and intricate organ, plays a pivotal role in maintaining overall body metabolism, facilitating movement, and supporting normal daily activities. An accumulating body of evidence suggests that microRNA (miRNA) holds a crucial role in orchestrating skeletal muscle growth. Therefore, the primary aim of this study was to investigate the influence of on myogenesis. In our study, the overexpression of was found to stimulate proliferation while suppressing differentiation in C2C12 myoblasts. Conversely, the inhibition of expression yielded contrasting effects. Through bioinformatics analysis, potential binding sites of with the 3'UTR region of BTG anti-proliferative factor 2 () were predicted. Subsequently, dual luciferase assays conclusively demonstrated as the direct target gene of . Further investigation into the role of in C2C12 myoblasts unveiled that its overexpression impeded proliferation and encouraged differentiation in these cells. Notably, co-transfection experiments showcased that the overexpression of could counteract the effects induced by . In summary, our findings elucidate that promotes proliferation while inhibiting differentiation in C2C12 myoblasts by targeting .