Yoo Yoonjeong, Lee Jihwan, Cho Jinho, Yoon Yohan
Department of Food and Nutrition, Sookmyung Women's University, Seoul, Republic of Korea.
Department of Poultry Science, University of Georgia, Athens, GA, USA.
Vet Med (Praha). 2023 May 29;68(5):191-199. doi: 10.17221/112/2022-VETMED. eCollection 2023 May.
This study aimed to use lactic acid bacteria isolated from piglet faeces to develop probiotics, allowing for the effective control of and . Lactic acid bacteria were isolated from the faeces of suckling piglets and identified by 16S rRNA sequencing, then examined for haemolysis; gelatinase activity; and resistance to acid, bile, and pancreatin. The antimicrobial activity of selected lactic acid bacteria isolates was examined for 8 and 7 strains. One-hundred and sixty-four lactic acid bacteria isolates were identified from 118 piglet faecal samples, and 13 lactic acid bacteria isolates were selected from analyses of haemolysis; gelatinase activity; and resistance to acid, bile, and pancreatin. Of the selected 13 lactic acid bacteria isolates, PF20-3 and PF30-3 strains had the highest antibacterial activity against and .
本研究旨在利用从仔猪粪便中分离出的乳酸菌开发益生菌,以有效控制和。从哺乳仔猪粪便中分离出乳酸菌,并通过16S rRNA测序进行鉴定,然后检测其溶血情况、明胶酶活性以及对酸、胆汁和胰酶的抗性。对选定的乳酸菌分离株进行抗菌活性检测,检测了8株和7株。从118份仔猪粪便样本中鉴定出164株乳酸菌分离株,并通过对溶血情况、明胶酶活性以及对酸、胆汁和胰酶的抗性分析,选出13株乳酸菌分离株。在选定的13株乳酸菌分离株中,PF20 - 3和PF30 - 3菌株对和具有最高的抗菌活性。