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在 中锰毒性胁迫下的全基因组鉴定和基因表达分析。

Genome-Wide Identification of and Analysis of Gene Expression under Manganese Toxicity Stress in .

机构信息

Department of Biotechnology, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.

Department of Agronomy, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.

出版信息

Genes (Basel). 2023 Nov 21;14(12):2109. doi: 10.3390/genes14122109.

Abstract

Malate dehydrogenase (MDH) is one kind of oxidation-reduction enzyme that catalyzes the reversible conversion of oxaloacetic acid to malic acid. It has vital functions in plant development, photosynthesis, abiotic stress responses, and so on. However, there are no reports on the genome-wide identification and gene expression of the MDH gene family in . In this study, the MDH gene family of was comprehensively analyzed for the first time, and 15 AhMDH sequences were identified. According to the phylogenetic tree analysis, are mainly separated into three subfamilies with similar gene structures. Based on previously reported transcriptome sequencing results, the expression quantity of roots and leaves exposed to manganese (Mn) toxicity were explored in . Results revealed that many were upregulated when exposed to Mn toxicity, suggesting that those might play an important regulatory role in 's response to Mn toxicity stress. This study lays foundations for the functional study of and further reveals the mechanism of the signaling pathway responding to high Mn stress.

摘要

苹果酸脱氢酶(MDH)是一种氧化还原酶,可催化草酰乙酸可逆转化为苹果酸。它在植物发育、光合作用、非生物胁迫响应等方面具有重要功能。然而,目前还没有关于. 中 MDH 基因家族的全基因组鉴定和基因表达的报道。在本研究中,首次对. 的 MDH 基因家族进行了全面分析,鉴定出 15 个 AhMDH 序列。根据系统发育树分析,. 主要分为三个亚家族,具有相似的基因结构。基于先前报道的转录组测序结果,探讨了. 根系和叶片在锰(Mn)毒性暴露下的表达量。结果表明,许多 在 Mn 毒性暴露下上调表达,表明这些 可能在 对 Mn 毒性胁迫的响应中发挥重要的调控作用。本研究为 的功能研究奠定了基础,并进一步揭示了. 信号通路响应高 Mn 胁迫的机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d8c/10743186/c13246a9e5b3/genes-14-02109-g001.jpg

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