一种用于检测线粒体过氧亚硝酸盐的小分子荧光探针。
A Small-Molecule Fluorescent Probe for the Detection of Mitochondrial Peroxynitrite.
机构信息
Institute of Optical Functional Materials for Biomedical Imaging, School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271016, China.
出版信息
Molecules. 2023 Dec 6;28(24):7976. doi: 10.3390/molecules28247976.
Reactive oxygen species (ROS) are pivotal signaling molecules that control a variety of physiological functions. As a member of the ROS family, peroxynitrite (ONOO) possesses strong oxidation and nitrification abilities. Abnormally elevated levels of ONOO can lead to cellular oxidative stress, which may cause several diseases. In this work, based on the rhodamine fluorophore, we designed and synthesized a novel small-molecule fluorescent probe () for ONOO. Upon reaction with ONOO, exhibited a significant fluorescence signal enhancement (approximately 34-fold). Moreover, showed an excellent mitochondria-targeting capability. Confocal fluorescence imaging validated its ability to detect ONOO changes in HeLa and RAW264.7 cells. Notably, we observed the ONOO generation during the ferroptosis process by taking advantage of the probe. displayed good biocompatibility, facile synthesis, and high selectivity, and may have potential applications in the study of ONOO-associated diseases in biosystems.
活性氧（ROS）是控制多种生理功能的关键信号分子。过氧亚硝酸盐（ONOO）作为 ROS 家族的一员，具有很强的氧化和硝化能力。异常升高的 ONOO 水平会导致细胞氧化应激，可能导致多种疾病。在这项工作中，我们基于罗丹明荧光团，设计并合成了一种新型小分子荧光探针（）用于检测 ONOO。与 ONOO 反应后，表现出显著的荧光信号增强（约 34 倍）。此外，它还表现出优异的线粒体靶向能力。共聚焦荧光成像验证了其在 HeLa 和 RAW264.7 细胞中检测 ONOO 变化的能力。值得注意的是，我们利用该探针观察到了铁死亡过程中 ONOO 的产生。表现出良好的生物相容性、易于合成和高选择性，可能在生物体系中与 ONOO 相关疾病的研究中有潜在的应用。
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