Genome-Wide Identification and Expression Analysis of the Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Gene Families in Rose ( cv ) under Different Light Conditions.

Trehalose, trehalose-6-phosphate synthase (TPS),and trehalose-6-phosphatase (TPP) have been reported to play important roles in plant abiotic stress and growth development. However, their functions in the flowering process of have not been characterized. In this study we found that, under a short photoperiod or weak light intensity, the content of trehalose in the shoot apical meristem of cv 'Carola' significantly decreased, leading to delayed flowering time. A total of nine and seven genes were identified in the genome. Cis-element analysis suggested that and genes were involved in plant hormones and environmental stress responses. Transcriptome data analysis reveals significant differences in the expression levels of and family genes in different tissues and indicates that and are potential key genes involved in rose flower bud development under different light environments. The results of quantitative real-time reverse transcription (qRT-PCR) further indicate that under short photoperiod and weak light intensity all members were significantly down-regulated. Additionally, , , and were up-regulated under a short photoperiod and showed a negative correlation with flowering time and trehalose content decrease. Under weak light intensity, was up-regulated and negatively regulated flowering, while , , , , and were down-regulated and positively regulated flowering. This work lays the foundation for revealing the functions of and gene families in the regulation of rose trehalose.