RNA 聚合酶 II 的全球调控。

Global control of RNA polymerase II.

机构信息

EMBL Australia Node in Single Molecule Science, University of New South Wales, Sydney, Australia; UNSW RNA Institute, University of New South Wales, Sydney, Australia; Department of Molecular Medicine, School of Biomedical Sciences, University of New South Wales, Sydney, Australia.

出版信息

Biochim Biophys Acta Gene Regul Mech. 2024 Jun;1867(2):195024. doi: 10.1016/j.bbagrm.2024.195024. Epub 2024 Mar 27.

DOI:10.1016/j.bbagrm.2024.195024

PMID:38552781

Abstract

RNA polymerase II (Pol II) is the multi-protein complex responsible for transcribing all protein-coding messenger RNA (mRNA). Most research on gene regulation is focused on the mechanisms controlling which genes are transcribed when, or on the mechanics of transcription. How global Pol II activity is determined receives comparatively less attention. Here, we follow the life of a Pol II molecule from 'assembly of the complex' to nuclear import, enzymatic activity, and degradation. We focus on how Pol II spends its time in the nucleus, and on the two-way relationship between Pol II abundance and activity in the context of homeostasis and global transcriptional changes.

摘要

RNA 聚合酶 II（Pol II）是负责转录所有蛋白质编码信使 RNA（mRNA）的多蛋白复合物。大多数基因调控研究都集中在控制何时转录哪些基因的机制上，或者集中在转录的机制上。Pol II 活性如何被确定受到的关注相对较少。在这里，我们从“复合物组装”到核输入、酶活性和降解，跟踪一个 Pol II 分子的生命历程。我们专注于 Pol II 在核内的时间分配，以及在稳态和全局转录变化的背景下，Pol II 丰度和活性之间的双向关系。