噬菌体匹配、治疗和监测方案，用于非缓解性感染中的同情性噬菌体使用。

Protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections.

机构信息

Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112102, Israel; The Israeli Phage Therapy Center (IPTC) of Hadassah Medical Center and the Hebrew University, Jerusalem 9112102, Israel; Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center (HHUMC), Jerusalem 9112000, Israel.

The Israeli Phage Therapy Center (IPTC) of Hadassah Medical Center and the Hebrew University, Jerusalem 9112102, Israel; Institute of Biomedical and Oral Research (IBOR), Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel.

出版信息

STAR Protoc. 2024 Jun 21;5(2):102949. doi: 10.1016/j.xpro.2024.102949. Epub 2024 Apr 30.

DOI:10.1016/j.xpro.2024.102949

PMID:38691464

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11070627/

Abstract

Phage therapy has re-emerged as a promising treatment for non-resolving infections. Given the lack of approved phage treatments, there is a need to establish a compassionate use pipeline. Here, we present a protocol for phage matching, treatment, and monitoring for compassionate bacteriophage use in non-resolving infections. We describe steps for consultation and request implementation, evaluating and comparing different aspects of phage activity, and phage production. We then detail procedures for multidisciplinary meetings, ethics approvals, phage therapy, and follow-up. For complete details on the use and execution of this protocol, please refer to Onallah et al..

摘要

噬菌体疗法作为一种有前途的非治愈性感染治疗方法重新出现。鉴于缺乏批准的噬菌体治疗方法，因此需要建立一个同情使用的途径。在这里，我们提出了一个用于非治愈性感染中同情使用噬菌体的噬菌体匹配、治疗和监测的方案。我们描述了咨询和请求实施、评估和比较噬菌体活性的不同方面以及噬菌体生产的步骤。然后，我们详细介绍了多学科会议、伦理审批、噬菌体治疗和随访的程序。有关本方案的使用和执行的完整详细信息，请参见 Onallah 等人的研究。